Philips CM12 manual Sample Image Adjustment-Optimizing Eucentric Z Height

Erwin Sabio Philips CM12 Alignment & Setup Manual Page 12

Sample Image Adjustment-Optimizing Eucentric (Z) Height

1.Adjust the beam intensity on the sample or increase the magnification by turning the magnification knob [14] to see certain structures of the sample. Then pick a feature on the center of the sample as a reference point for adjusting the Z-height.

Figure 14 The center of the four holes in this sample can be chosen as a reference point for z-height adjustment.

2.Unlock the tilt lever and tilt the sample from +10 degrees to -10 degrees to see how far the reference point moves. Return the sample to 0 tilt.

3.Turn the z-height knob [27] to one direction then tilt the sample +/-10 degrees again to check the movement of the reference point. If the movement becomes smaller, continue turning the knob in that direction. Otherwise, turn the other way.

4.Continue alternating between turning the z-knob and tilting the device until the movement of the reference point reaches a minimum. At this point, the optimal z- height has been reached.

5.Return the tilt to 0 degree then put the lever in lock position.

Sample Image Adjustment-Adjusting Rotation Center

1.As in z-height adjustment, begin by choosing a central feature or the sample as a reference point.

2.Press the ALGN button on the control panel and select ROT CENTER from the onscreen menu.

3.The image should wobble in and out i.e. symmetrically around the center when the objective rotation center is aligned. If the reference point moves in addition to the wobbling, adjust the multifunction X/Y knobs until the reference point stays put.

4.Another way to check if the rotation center is aligned is to got to different focus steps by turning the outer knob of the focus-focus step knob [20]. The wobbling of the sample should appear relatively the same at different focus steps.