Instructions 71-5000-15 AD Pre-activated media

GE Healthcare

CNBr-activatedSepharose 4 Fast Flow

Introduction

The preparation and us e of affinity ch romatography me dia by coupling bios pecific
ligands to CNBr-activa ted matrices is a wi dely used, success ful and well-do cumented
technique.
CNBr-activated SepharoseTM 4 Fast Flow is a pre-activated affinity matri x that
combines the advantag es of CNBr coupling wi th the high flow an d stability
characteristic s of Sepharose 4 Fast Fl ow. In our experienc e, the CNBr coupling
technique has a well-p roven track record for t he purificati on of therapeutic
proteins. This, plus t he performanc e of the matrix at larg e scale, makes the us e of
CNBr-activated Sepha rose 4 Fast Flow par ticularly at tractive for manu facturing
applications in the b iopharmaceut ical industry. Fu rthermore, th e medium is a
member of the BioProces sTM media. BioProcess m edia are develope d and supported
for production scale c hromatography . All BioProcess me dia are produced wit h
validated methods an d are tested to meet ma nufacturing re quirements. Se cure
ordering and deliver y routines give a reli able supply of medi a for production
scale. Regulatory Sup port Files (R SF) are available to a ssist process val idation and
submissions to regulatory authorities.
To ensure best performa nce and trouble-f ree operation, pl ease read these
instructions be fore using CNBr-acti vated Sepharose 4 Fa st Flow.