Hach HCT 159 10 5.00 mg/L Free-Fe / 0.12 6.00 mg/L Tot-Fe, UniCell HCT, Procedure for Total Iron

HCT 159

PROCEDURE for Free Iron

applies to DR/4000, DR/2500 DR/2010 and DR/800

DR / 4000—Rev 2.39

HCT 159

MEASURING RANGE:

0.10 - 5.00 mg/L Free-Fe / 0.12 - 6.00 mg/L Tot-Fe

1

2

4.0ml

Sample

1. Enter program number and set up the instrument as explained for each instrument type.

2. Pipet 4.0 ml sample into the sample vial.

Note

If this is the first time you have run this revision of the test on your DR/4000, you must contact your local Hach distributor for an upgrade of your software (Cat.No. 49544-00). Software Version number 2.39 contains the factory UniCell™ programs.

INSTRUMENT SET-UP

1.	Press the soft key under HACH PROGRAM (or
	USER PROGRAM if the method was entered as a
	USER PROGRAM).
	Enter program number 2130 (or 159 if the method was
	entered as a USER PROGRAM).
	Press ENTER.
2.	The display will show:
	HACH PROGRAM: 2130
	Iron, HCT 159
	The wavelength ( λ), 485 nm, is automatically selected.
3.	Insert the Test N’Tube adapter into the cell holder by
	sliding it under the thumb screw and into the alignment
	grooves. Fasten in place by tightening the thumb screw.
4.	Follow the procedure steps.
	(Note: If measuring Total Iron and performed the sample
	digestion using the HCT 200 Metal Prep Set, select the

3

415min

3. Close sample vial and invert a few times, until the freeze-dried contents are completely dissolved.

4. Start the instrument timer.

A 15-minutereaction period will beginn.

	Total Iron alternate form under the Options soft key menu).
5.	Wipe the outside of the zero vial.
	Place the zero vial into the adapter.
	Close the light shield.
6.	Press the soft key under ZERO.
	The display will show: 0.00 mg/L Free-Feor

0.00 mg/L Tot-Feif the Total Iron alternate form was selected.

7.Clean the outside of the sample vial.

8.Place the prepared sample vial into the adapter. Close the light shield. Results in mg/L Free-Feor Tot-Feare displayed.

Note

If the test overranges, repeat the UniCell™ test with diluted sample.

5

ZERO

5. Zero the instrument using the zero vial (white cap) provided and read the sample.

LIST OF REQUIRED REAGENTS
Iron-Fe
UniCell™ HCT 159	23/pkg	HCT 159
OPTIONAL REAGENTS
Metal Prep Set
UniCell™ HCT 200	50 digestions	HCT 200
Iron Standard
1000 mg/L as Fe	100 ml	2271-42
Sodium Hydroxide
Standard Solution	1000 ml	2450-53
(5.0 N)
Nitric Acid, concentrated	500 ml	152-49

PROCEDURE for Total Iron:

After digestion with the Metal Prep Set HCT 200, follow the procedure steps 1 – 5.

OPTIONAL APPARATUS
Cylinder, mixing – 100 ml	each	20886-42
Flask, volumetric 100 ml	each	547-42
Pipettor, (Jencons) 1-5 ml	each	27951-00
Replacement tips for 27951-00	pk/100	27952-00
Pipettor, (Jencons) 100-1000 µl	each	27949-00
Replacement tips for 27949-00	pk/400	27950-00
pH Paper	pk/100	26013-00