Sanyo MDF-C2156VANC VIP Plus Design, Specially Designed Compressor for Cryogenic Temperatures

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-150°C CryogenicTemperature Freezer

Cryopreservation allows for storage of biological materials at ultra low temperatures, while minimizing the level of freezing damage to cells. An important factor to consider when preserving cells or tissue at cryogenic temperatures is to prevent amorphous ice crystals from recrystallizing within and outside the cells. These ice crystals increase the degree of freezing damage to cells. -130°C is the recrystallization point of pure water in the ultra-low temperature zone. This is the temperature at which amorphous ice crystals recrystallize. For a mixed solution containing Me2SO and other cryoprotectants, recent research confirms that recrystallization occurs around -115ºC. Thus samples maintained in an ultra-low temperature freezer at -150ºC, far lower than the recrystallization point, can be semi permanently preserved. Such preservation maintains vitrification without further crystallization within and outside cells. Other recent findings show that preserving cattle sperm at -135°C is insufficient, and also that superconductivity experiments

require temperatures of at least -148ºC. These cases show the increased necessity of -150ºC freezing in a diverse array of cryogenic applications.

VIP® Plus™ Design

•SANYO V.I.P.® PLUS™ Cryogenic Series -150°C ultra-low temperature freezers use patented revolutionary vacuum insulation cabinet construction that reduces the wall thickness from 175mm to 135mm (6.9” to 5.3”) and achieves up to 25% more storage capacity than a conventionally insulated freezer

without increasing the footprint.

•This design prevents the insulation layers from distortion and cracking that might occur due to temperature differences inside and out, and creates the most efficient insulation material available today.

•Moisture condensation at the top edges of the cabinet due to differences in temperature inside and out causes frost and icing problems, which may reduce heat insulation efficiency and obstruct door movements. These

obstructions are prevented by the “hot line” which prevents frost and condensation from forming on the gasket and mullion

•The newly developed refrigeration system and the freezer structure offer a quiet experimental environment.

• Achieves 50% higher energy conservation than conventionally insulated model.

Specially Designed Compressor for Cryogenic Temperatures

The refrigeration system is at the heart of producing cryogenic temperature freezing. In the process of developing various technical solutions in order to achieve and maintain cryogenic temperatures, SANYO designed the compressor specifically for this use. SANYO is a leading manufacturer of cryogenic temperature freezers.

Liquid Nitrogen Vapor Storage Mechanically Refrigerated Cryogenic Freezer

Comparison of temperature distribution in a liquid nitrogen freezer (vapor phase) and SANYO’s MDF-C2156VANC mechanically refrigerated cryogenic freezer. Graph shows temperatures at different locations within the chamber. This data demonstrates that 100% of the MDF-C2156VANC storage space maintains uniform storage temperatures safely below -130ºC, while temperature in LN2 vapor system is dependent on storage location.