Olympus Microscope manual Classification of Aberrations, Astigmatism

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OPTICAL TERMINOLOGY

9. Aberrations

A difference between an ideal image and an actual image that passes through an optical system is called an “aberration.”

9.1 Requirements for Ideal Image Formation

The following three requirements must be satisfied to form an image with no aberration, or an ideal image.

(i)All the light rays coming from a single point and passing through an image formation optical system converge on a single point.

(ii)Image points, which correspond to object points on the same plane perpendicular to the optical axis, are present on the same plane.

(iii)The planar shape of an object and the planar shape of an image that are on the same plane perpendicular to the optical axis have a similarity relation.

Figure 9-1 Requirements for Ideal Image Formation

i

ii

iii

Object Image plane

 

 

In an actual optical system, however, it is very difficult to strictly meet the requirements for ideal image formation and this causes “aberrations” that interfere with image forming performance.

9.2 Classification of Aberrations

Aberrations that interfere with image forming performance are classified as shown below in Figure 9-2.

Seidel’s aberration = “Expansion of a point image” + “Curvature of image plane” + “Deformation”

Figure 9-2 Classification of Aberrations

characteristics of glass materials used for the optical system. “Expansion of a point image” can also be expressed by “wavefront aberration” that regards the light as “waves” and takes account of the phase to include the influence of diffraction.

(1) Spherical aberration

When light rays coming out of an axial object point enter a lens, the light rays with a larger numerical aperture (N.A.) are subjected to stronger refraction power and cross the optical axis in positions with larger differences from the ideal image formation position. The aberration caused this way by different image forming positions due to differences in N.A. of axial light rays is called “spherical aberration.” (“Spherical aberration” is proportional to the cube of N.A.)

Figure 9-3 Spherical Aberration

 

Specimen

Aplanatic

 

 

tube lens

 

Objective lens with

Image plane

spherical aberration

 

It is said that objective lenses with larger N.A. have better resolution but worsen spherical aberration. Our advanced design and manufacturing techniques have realized good optical performance even with large numerical aperture.

(2) Coma aberration

Even though spherical aberration is compensated to be very small, there are cases where light rays coming out of an off-axis object point are not condensed to a single point on the image plane but generate asymmetric blur just like a comet leaving traces. This is called coma aberration.

Figure 9-4 Coma Aberration and Spot Shape on the Image Plane

Aberration

Seidel's aberration

Chromatic aberration

(1) Spherical aberration

(2) Coma aberration

(3) Astigmatism

(4)Field curvature

(5) Distortion

(6)Longitudinal (axial) chromatic aberration

(7)Chromatic aberration of magnification

Specimen

Aplanatic

 

 

tube lens

 

Objective lens with

Image plane

coma aberration

 

 

(3) Astigmatism

Types (1) to (3) correspond to “expansion of a point image” that goes against requirement (i) for ideal image formation in Figure 9-

1.Type (4) corresponds to “curvature of image plane” that goes against requirement (ii) in Figure 9-1. Type (5) corresponds to “deformation” that goes against requirement (iii) in Figure 9-1. Types (6) and (7) correspond to “color blur” of images caused by

Even though a lens is compensated for spherical aberration and coma aberration, there are cases where an image of an off-axis object point is not focused to a single point but separated to a concentric line image and a radial line image. This is called “astigmatism.” When astigmatism is present, a point image blurs vertically and horizontally, before and after the focus position.

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Contents Microscope Components Guide 0 6 1Page Contents UIS/UIS2 TLUFeatures of UIS2 objective lenses Video system Illumination systems and power supplyFocusing units Observation/single tubesMotorized Unit System Diagram DIC*1 POL UIS2LCD UIS2 MplflnPlan SemiApochromat Mplfln seriesLong WD M Plan SemiApochromat Lmplfln seriesPlan Achromat Mpln seriesLCPLFLN20xLCD LCD Long WD M Plan SemiApochromatLCPLFLN-LCD series LCPLFLN50xLCDMPlanApo series Plan ApochromatSuper Long WD M Plan Achromat SLMPlan seriesIR Long WD M Plan SemiApochromat/IR M Plan SemiApochromat LMPlan-IR series/MPlan-IRPlan SemiApochromat BD MPLFLN-BD seriesPlan SemiApochromat BDP MPLFLN-BDP seriesLong WD M Plan SemiApochromat BD LMPLFLN-BD seriesPlan Achromat BD MPLN-BD seriesMPlanApo-BD Focal distance WeightPlan Apochromat BD MPlanApo100xBD32H8 Bxfm frameBXFM-F HoleBxfm BXFM-F+BXFM-ILH+BXFM-ILHSPUBXFM-S BXFM-F+BXFM-ILHSUniversal stand type StandsSZ2-STU2 SZ-STLBX-RLA2 Reflected light illuminator for BF/DFUniversal reflected light illuminator BX-URA2Less Reflected light illuminators for BFBX-KMA/BX-KMA-ESD ThanKmas Reflected light illuminator for BFVery compact reflected light illuminator with reduced depth LH100HGAPO Lamp housingsLH75XEAPO LH100HGRMT Lamp housing accessoriesTH4-HS RCV Lgad SZX-TLGADLG-SF LG-PS2Dulha TR30IR Widefield trinocular observation tubesSingle port tube with lens TLUMX-SWETTR Super widefield trinocular observation tubesSWTR-3 Swetr Super widefield erect image tilting trinocular tubeECA Various accessories for various observation needIntermediate tubes TRUEPA2 APTOSM EyepiecesFilar micrometer eyepiece Eyepieces for UIS2 optical systemD6RE-ESD Revolving nosepieces for BF objective lensesD6RE D7RE P4REP5BDRE Revolving nosepieces for BF/DF objective lenses5BDRE D5BDRE D6BDRE BD-M-ADTV0.5xC-3 Mount video port with 0.5x lens Mount video camera portsTV0.35xC-2 TV0.63xC Mount video port with 0.63x lensCMAD3 Bmad Video camera mount adaptersVideo camera port Tmad FMTBX-RLAA+U-D6REMC+U-LH100-3 Motorized unitsBX-RFAA P5REMC D5BDREMCD6REMC BX-UCBActive auto focus unit AFA1MFWR BXFMA-F Focus adjustment knob unitFocus adjustment knob interface IffhUVF2FB/5FB Deep ultraviolet observation systemUVF248IM UVF248LB+U-LH80HGXEFocal length of tube lens Observation through eyepiece binocular observation3355 = 50 ⋅ 0.5 =352µmMPLFLN100⋅ N.A.=0.90, λ =0.55µm Focal depth formula Visual observation Berek formulaClassification of Aberrations AstigmatismAberration of magnitude DistortionWavefront Aberration Olympus Corporation has obtained ISO9001/14001