Olympus AX70 manual Resolution Contrast Imaging

RESOLUTION AND

CONTRAST IMAGING

Bright, high-quality fluorescence images.

The AX optical system attains the very best brightness and assures crystal- clear fluorescence images by employing an improved numerical aperture and high transmission objectives and by incorporating a 100% optical path and high transmission optics. The ideal mirror unit for each application can be selected from a range of 18. The built-in illuminator accepts up to four mirror units, allowing the user to create the ideal combination for each purpose or for a combination of multiple observation methods. In addition, the motorized excitation light shutter also minimizes fluorescence fading.

Impeccable phase-contrast observation.

The AX Series features new phase- contrast objectives employing an optimally positioned innovative low-

reflection phase membrane. Appropriate phase-contrast and transmission are ensured for a wide wavelength range for outstanding contrast and image quality.

Nomarski DIC with higher contrast and resolution.

Sophisticated manufacturing technology and new multi-coatings for the high-performance Universal Apochromat objectives guarantee superb optical performance for fluorescence and Nomarski DIC observations. Nomarski prisms specially developed for Video Enhanced Contrast and metallurgy are also available.

Design algorithms suited for polarized light observation.

The entire UIS optical system including high-precision, strain-free POL-objectives, condensers and observation tube prisms has been designed to minimize reflections from

lens surfaces and frame. Together with dedicated Olympus optical design algorithms, this ensures unmatched image contrast for polarized light observation. Used with the Brace Koehler compensators, these objectives allow high-sensitivity examination of biological samples.

Live cell imaging.

The special correction of the UPLAPO60×WPSF objective renders symmetrical image information from planes above and below the focal plane.

This imaging symmetry makes it the objective of choice of digital deconvolution applications. The water immersion correction of the objective eliminates spherical aberration when imaging live material, even when observing deep within a cell（up to 250∝ m）. At the same time, the objective's high numerical aperture（N.A. 1.20）achieves spectacular resolution.

Defocus images of the point spread function

General objectives

Non symmetry of image information from the planes above and below the focus level as obtained with well corrected objectives.

PSF objectives

The special correction of the UPLAPO60×WPSF objective renders symmetrical image information from planes above and below the focal plane.

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