Leica Understanding Photo Tubes and C-mounts for Optimal Microscope Performance

Page 45

Photo Tubes and C-mounts

Application

All Leica DFC cameras are equipped with a standardized C-mount interface. In turn, the C-mount adapter for the respective trinocular tube is connected to this interface. This adapter creates a solid mechanical connection between the microscope and camera and ensures opti- mum rendering of the microscopic image on the image sensor of the camera.

Usually, the ideal is for the digital camera to capture as much of the field of vision as possi- ble, while excluding as much of the black edge of the field of vision as possible. To do so, the magnification factor of the C-mount adapter must match the image format of the sensor as closely as possible (see table).

If there is unwanted shading at the corners even with a compatible C-mount adapter installed, it can be corrected using the "Shading function" of the camera software.

Alternatively, you can also use a C-mount adapter with higher magnification. This primar- ily avoids the critical border area of the field of vision and concentrates on the center of the field of vision.

Camera

optimal

suitable

 

(large

(smaller

 

image field)

image field)

 

 

 

DFC295

0.5×

0.63×

DFC290 HD

 

 

 

 

 

DFC425

0.5×

0.63×

DFC425 C

 

 

 

 

 

DFC495

0.63×

0.8×

 

 

 

DFC500

0.63×

0.8×

 

 

 

Cameras from third-party suppliers

In addition to Leica DFC cameras with the stan- dardized C-mount interfaces, you can connect third-party cameras to the microscope using a T2 bayonet adapter. To do so, instead of the C-mount adapter, simply use the correspond- ing SLR adapter with T2 connection. However, these third-party cameras are not integrated into the Leica Application Suite and have to be operated using the corresponding software from the camera manufacturer.

The Leica digital cameras are detailed in a sepa- rate user manual along with instructions for their assembly and use.

Leica M-series

User Manual

45

 

 

 

Image 45 Leica M80, M60 user manual Photo Tubes and C-mounts, Application, Camera Optimal Suitable, DFC295 63×
Contents Leica M50 Leica M60 Leica M80 General Instructions Important Safety Notes Important information Symbols UsedProvide clarity Explanatory notesDescription Safety RegulationsDesignated use Non-intended useSafety Regulations contd During maintenance work on the supply unit UV radiation could damage the eyes. Therefore­Supply unit Mercury lamp Lamp housingTable of Contents Inhaltsverzeichnis Leica M50 Leica M60 Leica M80 Different Types of ObjectivesLeica M series Congratulations New Leica M SeriesMagnification in stages or zoom Have a special request? Let us know Modular Design Everything is RelativeObjectives Maximum CompatibilityTubes EyepiecesOn We Go Assembly Base and Focusing Column Assembling the column adapterTools used Assembly Using the screw providedMicroscope Carrier Tools used Assembling the optics carrier None Optics CarrierFor the optics carrier Tighten the clamping screw below the optics carrierNo tools required TubePreparation Assembling the tubeEyepieces Tools used PreparationMagnification range Attaching the objective ObjectiveLeica LED5000 CXI Coax Illumination Alternative installation for manual columnsRequired tools NoneLeica LED5000 CXI contd With any objectives that have an outer Leica LED3000 NVI Near Vertical IlluminationLeica LED3000 NVI contd Quick Start Guide Take time to read it its worth it Reliable Route to SuccessOverview of an M Series Microscope Correct Interpupillary Distance Adjusting the interpupillary distanceReference value Look into the eyepiecesDioptric correction Using the EyepiecesIf you do not wear glasses If you wear glassesFocusing Adjusting the Resistance of the Focus Drive Adjusting the resistanceFocusing­ Changing the Magnification Zoom ZoomingLook into the eyepieces Focus on the specimen Ratchet Steps and Magnification Levels Enabling and disabling ratchet stepsMagnifications and fields of view Eyepieces & Tubes Following eyepieces are available for the M series Magnification Factor of the EyepiecesPotential sources of infection Health NotesDioptric Correction Using the Dioptric CorrectionDioptric Correction and Parfocality AdjustingUse GraticulesErgoTube 10 to ErgoTube 10 toHandling Photography & Video Adapter Photography & VideoLeica DFC cameras Leica Application SuiteApplication Photo Tubes and C-mountsCamera Optimal Suitable DFC295 63×Separation ratios Video/Phototubes HD F & HDObjectives and Optical Accessories Achromatic objectives Different Types of ObjectivesPlanachromatic objective 1× Achromatic objectives with a long focal lengthSystem Illumination Leica LED3000 NVI Near Vertical Illumination LAS or the Leica SmartTouch Be very bright. Therefore, always switchNot a malfunction ButtonsDimensional Drawings Leica M50 Leica M50 with large incident-light base and ErgoTube 278 514 116 301 119 255 436 322278 260 500 173 170 709 Leica M60 Leica M80 500 606 106 356 154 420 290 430 340 220 Leica M80 with Leica LED2500 and binocular ErgoTube 631 369 28.5 600 290 Max Appendix Parameter ExampleCalculation example magnification in binocular tube Warranty benefits Care, Maintenance, Contact PersonsContact address Mail contactCleaning polymer components Care, Maintenance, Contact Persons contdProtection from dirt Permitted measures