Nikon L-Ke manual Focusing

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Manipulate two centering screws until the image of the illumination field diaphragm comes to the center.

You need not mind about a slight deviation from this centering caused

by switching over to other objectives because this will not produce any objectionable result.

6.Observation

For usual case of observation with objectives from 4X to lOOX (oil-immer- sion), it suffices to leave the change-over lever at the position L. When an exact illumination or extreme brightness is required, as is the case with photomicrography, it is necessary to make adjustment for each magnifi- cation by switching over the lever.

7.Photomicrography

Make exact adjustment, depending upon the magnification of the objec- tive being used, by switching the change-over lever as below:

Objective

magnification

4X

1 0 - 2 0 X

 

40 - lOOX

Position

of the change-over lever

L

M

i

H

 

 

 

 

i

 

When using an objective 3X or lower, set the change-over lever to H and replace the condenser with the one specifically designed for lower magnification.

The illumination for the Model L-Ke will not change its color temper- ature by the change-over above mentioned.

8.Other Light Sources

When using any other light source outside of the microscope, replace the illumination field lens with the accessory reflecting mirror, as shown in Fig. 12.

9.Filter Case

A filter of the diameter 45mm can be mounted on the illumination field

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lens. A filter of the diameter 33mm can be placed in the condenser or in the filter case of the microscope. This filter case to be attached onto the microscope base is available on order.

FOCUSING

1.Focusing Adjustment

The microscope model L-Ke is provided with coaxial, coarse and fine focusing knobs, both of which are located near the microscope base (Fig.

13). Forward rotation of either of the focusing knobs by the operator raises the microscope stage and vice versa (Fig. 13, 14).

2.Eyepiece Adjustment

When using a binocular or trinocular eyepiece tube for observation the adjustment of the user's eye-sight (diopter) discrepancy between the right and left eyes is necessary which is made by rotating the adjusting ring on the lefthand eyepiece.

After focusing with your right eye by raising or lowering the microscope stage, turn the adjusting ring left or right to obtain the sharp image with your left eye, too. Then, regulate the interpupillary distance of the bino- cular or trinocular tube by sliding the eyepieces left or right by means of the knob (Fig. 15), until the viewfields of both eyepieces merge together. It will be advantageous to memorize the attained diopter and inter- pupillary distance readings for future use.

The red dot engraved on the interpupillary distance scale indicates the position where the mechanical tube length becomes exactly 160mm. The HK (high eyepoint type) eyepieces have an eyecup on top, the rotation of

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Image 9
Contents Nikon Contents Nomenclature Model LBR-Ke Various Combinations Interchangeable Eyepiece TubesInterchangeable Stages OBJECTIVES, EYEPIECES, Condensers 1. Objectives Attaching the Lenses CondensersMounting the Objectives Mounting the Eyepieces IlluminationIVIounting the Condenser Condenser Iris Diaphragm and ImagesBrightness Adjustment Condenser Focusing KnobPreparation and Adjustment Light SourceFocusing Preset Device Coarse FocusingFine Focusing Oil ImmersionO n g W o r k i n g R a n g e of t h e S t a g e Moving the Specimen on the StagePhotomicrography Exchanging Objective Nosepiece