p. 11
or
Wash the column with 3– column vol umes of 70%
ethanol. Wash immediately w ith at least 5 colu mn
volumes of sterile filtere d binding buffer.

Sanitization

Sanitization inac tivates microbi al contaminants i n the packed column an d
related equipment. A s pecific sanit ization protoco l should be designe d for
each process according t o the type of contamina nts present and st ability of
coupled ligand. Foll owing are generall y recommended pro cedures.
Equilibrate with a buffer consisting of 2% hibitane digluconate and 20%
ethanol. Allow to sta nd for 6 hours, then wa sh with at least 5 co lumn
volumes of sterile bin ding buffer.
or
Equilibrate with 70% ethanol. Allow to stand for 12 hours, then wash with at
least 5 column volumes o f sterile bindin g buffer.
Note: Specific regula tions may apply wh en using 70% ethano l since it can
require the use of explos ion-proof areas a nd equipment.

Storage

CNBr-activated Sepha rose 4 Fast Flow is sup plied freeze drie d. Additives are
included to preserve t he bead form of the me dium. When store d below 8 °C,
the shelf life is at leas t 18 months. Packed col umns should prefer ebly be
equilibrated in binding buffer containing 20% ethanol (ligand dependent) to
prevent microbial grow th.
6. Ordering information
Product Pack size Code No
CNBr-activated Sepha rose 4 Fast Flow 10 g 17-0981-01
250 g 17-0981-03
2 kg 17-0981-05
All products are supp lied freese drie d in the precence of add itives.