GE RPN2510E, RPN2511E, RPN2512E Appendix 3. Radioactive labeling systems

Appendix 3. Radioactive labeling
systems
Labelling Technology Nucleotide Amount of Labelling Probe specific Recommended Product
system template time activity application code
(dpm/µg)
Rediprime ™ II Random-prime dCTP only 25 ng 10 minutes 2 × 109 membrane RPN1633
hybridization RPN1634
Ready-To-Go ™ Random-prime dCTP only 10 ng–1 µg 5 minutes 2 × 109 membrane 27-9240-01
DNA labelling hybridization
beads DNA labelling
Megaprime ™ Random-prime any dNTP 25 ng 10 minutes 2 × 109 membrane RPN1604
hybridization RPN1605
RPN1606
RPN1607
Nick translation Nick translation any dNTP 1 µg 2–3 hours 2 × 109 production of N5000
large amounts N5500
of probe
RPN2510EUM Appendix 3 Rev C 06/2007 16