Leica MZ16 F user manual Double iris diaphragm, Observation without fluorescence

5.1.1 Observation without fluorescence

The FLUOIII® filter system includes a UV shutter that can be used to close the illumination beam path. This feature protects the mercury lamp, which should not be switched on and off too fre- quently. If, for instance, you need to interrupt your work, you do not have to switch off the high-pres- sure mercury burner to preserve sensitive speci- mens from being burned out or bleached out. Switching the high-pressure mercury burner on and off frequently decreases its life and causes delays, as the lamp can only be switched back on after it has cooled. Instead, you can close the UV shutter and reopen it when you return to your workplace.

Close the UV shutter:

–if you do not want to view the specimen with fluorescence illumination, but with transmitted light only.

–if you are taking a short break from work.

￿Close the illumination beam path using the switch (7.1).

5.1.2 Double iris diaphragm

You can use the built-in double iris diaphragm to adjust the depth of field individually. When doing so, however, note that when you increase the depth of field,

–the brightness decreases, and therefore the exposure times for photography increase,

–the resolution decreases.

￿Adjust the double iris diaphragm as necessary using the knurled ring (8.1).

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Fig. 7

1On/off switch for UV shutter

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Fig. 8

1Double iris diaphragm

Leica MZ16 F – Functions 19