Instructions for USE ‑ Readout Procedure, Accept Screen

Setting the Incubation Well Configuration

Incubation wells 1‑5 and 6‑10 can be configured to run the same or different types of 3M™ Attest™ Super Rapid Readout Biological Indicators. The flashing “A” in Attest indicates the setting may be changed by the user. There are three incubation well configuration options:

LCD Panel Configuration		Result
Setting

Attest 1491	Attest 1492	Auto‑reader wells 1 ‑ 5 configured for 1491 BIs
		(blue cap, 30 minute readout) and wells 6 ‑ 10
		configured for 1492V BIs (brown cap, 1 hour
		readout)

Attest 1491	Attest 1491	Auto‑reader wells 1 – 10 configured for 1491 BIs
		(blue cap, 30 minute readout)

Attest 1492	Attest 1492	Auto‑reader wells 1 – 10 configured for 1492V BIs
		(brown cap, 1 hour readout)

Use the Up Arrow or Down Arrow buttons to move between the three options until the desired configuration setting is displayed.

Press to select the desired option and advance to the Accept screen.

Note 1: If there are any incubating BIs which do not yet have a final result (+ or ‑) displayed, the well configuration for the occupied wells will NOT change until the final result is displayed on the LCD panel and the BI is removed from the reader.

Note 2: Ensure the color‑coded incubation well configuration sticker surrounding the incubation wells matches the programmed well configuration.

Accept Screen

Use the up or down buttons to toggle between Yes and No, press to accept changes (Yes selected) or retain current settings (No selected) and exit the configuration mode. The LCD panel will return to the standard display.

INSTRUCTIONS FOR USE ‑ Readout Procedure

Instructions for Biological Indicator (BI) Activation and Incubation

CAUTION: Allow the 3M™ Attest™ Super Rapid Readout Biological Indicator to cool for the recommended time period before activating. Activating or excessive handling of the biological indicator before cooling may cause the glass ampoule to burst.

CAUTION: To avoid the possibility of the 3M™ Attest™ Super Rapid Readout Biological Indicator vial absorbing fluorescent residue from a chemical indicator or tape, place the 3M™ Attest™ Super Rapid Readout Biological Indicator vial so it does not come in direct contact with chemical indicators or tape.

WARNING: Match the cap color of the 3M™ Attest™ Super Rapid Readout Biological Indicator with the color‑coded configuration sticker surrounding the 3M™ Attest™ Auto‑reader 490 incubation wells.

CAUTION: Wear safety glasses when activating the 3M™ Attest™ Super Rapid Readout Biological Indicator.

Follow this procedure for every 3M™ Attest™ Super Rapid Readout Biological Indicator to be read:

While wearing safety glasses, PLACE 3M™ Attest™ Super Rapid Readout Biological Indicator (BI) in color‑coded 3M™ Attest™ Auto‑reader 490 well. Note that BI vial is keyed for specific orientation in well (flat back of BI faces back of 3M™ Attest™ Auto‑reader 490).

CLOSE BI cap and CRUSH media ampoule by firmly pushing vial straight down in incubation well.

Immediately remove BI, visually VERIFY the glass ampoule (containing growth media) has been crushed, and FLICK BI to ensure purple growth media flows to the growth chamber at the bottom of the vial.

Note: If the audible alarm is enabled it will sound when the BI is removed from the incubation well if the reader has begun incubation countdown.

RETURN the crushed BI to the 3M™ Attest™ Auto‑reader 490 well and observe 3M™ Attest™ Auto‑reader 490 LCD panel to confirm correct placement of the BI. If the BI is correctly seated, the 3M™ Attest™ Auto‑reader 490 LCD panel will display ***, **, * and then remaining minutes of incubation directly below the incubating BI.

RECORD BI result.

WARNING: Do not remove the 3M™ Attest™ Super Rapid Readout Biological Indicator from the incubation well until the (+) or (‑) symbol on the LCD panel indicates the test is complete.

INSTRUCTIONS FOR USE – Additional Information

BI Placement

Wells are configured and color coded to match the cap of the 3M™ Attest™ Super Rapid Readout Biological Indicator i.e., the 3M™ Attest™ Super Rapid Readout Biological Indicator 1491 with a blue cap goes into a blue well and the 3M™ Attest™ Super Rapid Readout Biological Indicator 1492V with a brown cap goes into a brown well.

Remaining incubation time, in minutes, will be displayed on the LCD display to indicate the 3M™ Attest™ Super Rapid Readout Biological Indicator is incubating in the reader well and should not be touched. The time remaining display indicates the results are not available and incubation is in progress.

Do not remove 3M™ Attest™ Super Rapid Readout Biological Indicators until the

(+) or (‑) symbol on the LCD panel indicates the test is complete.

Note: If 3M™ Attest™ Super Rapid Readout Biological Indicators are inadvertently removed before the incubation period is complete, an alarm will sound and a C2 Caution code will also be displayed on the LCD panel under that well. You must return the 3M™ Attest™ Super Rapid Readout Biological Indicator to the reader well within 10 seconds or the results will be lost.

WARNING: Do not remove or change placement of 3M™ Attest™ Super Rapid Readout Biological Indicator once it is placed into a well. Doing so may result in loss of results and the 3M™ Attest™ Super Rapid Readout Biological Indicator test may be invalid.

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