Agilent Technologies 1100 Series manual Priming and Purging the System

Page 28

Installing the Pump

Priming and Purging the System

Priming and Purging the System

The system can be primed either by drawing solvent through the degasser with a syringe or by pumping with the pump.

Priming the system with a syringe is recommended, when:

•vacuum degasser or connected tubings are used for the first time or vacuum tubes are empty or

•changing to solvents that are immiscible with the solvent currently in the vacuum tubes.

 

Priming the system by using the pump at high flow rate

 

(3–5 ml/min) is recommended, when:

 

• pumping system was turned off for a length of time (for example,

 

overnight) and if volatile solvent mixtures are used, or

 

• solvents have been changed.

 

 

WA R NI N G

When opening capillary or tube fittings solvents may leak out. Please

 

observe appropriate safety procedures (for example, goggles, safety

 

gloves and protective clothing) as described in the material handling

 

and safety data sheet supplied by the solvent vendor, especially when

 

toxic or hazardous solvents are used.

 

Priming with a Syringe

 

 

Before using a new degasser or new tubings for the first time:

1Prime all tubings with at least 30 ml of iso-propanol no matter whether the channels will be used with organic mobile phase or with water.

If you are changing to a solvent that is immiscible with the solvent currently in the tubing continue as follows:

2Replace the current solvent with adequate organic solvent (see Table 5 on page 30), if current solvent is organic or with water, if current solvent is an inorganic buffer or contains salt.

3Disconnect solvent outlet tube of the channel that is supposed to be primed from your pump.

4Connect syringe adapter to solvent outlet tube.

28

Page 27 Page 29
Image 28
Page 27 Page 29
Contents Reference Manual Warranty Agilent 1100 Series Quaternary Pump This Book Installing the Pump Status IndicatorsError Messages Pressure Test Leak TestExchanging Internal Parts 127 Overview of Main AssembliesCable Overview 181 Introduction to the Quaternary PumpControl Module Screens for the Quaternary Pump Contents Contents Installing the Pump Power Cords Power ConsiderationSite Requirements Bench Space EnvironmentPhysical Specifications Type Comments Delivery Checklist Quaternary Pump Checklist Description QuantityUnpacking the Quaternary Pump Damaged PackagingAccessory Kit Contents Quaternary Pump Accessory Kit Contents-Vacuum Degasser Optimizing the Stack Configuration Recommended Stack Configuration Front ViewStack containing the autosampler Preparations Installing the Quaternary PumpParts required Rear of Quaternary Pump Settings, see Setting the 8-bit Configuration Switch on Front panel is turned offFlow Connections of the Quaternary Pump Two wrenches 1/4-5/16 inch for capillary connectionsToxic or hazardous solvents are used Removing the Front CoverFlow Connections of the Quaternary Pump Mcgv Priming and Purging the System Priming with the Pump Activity Solvent Comments Optimizing Performance Hints for Successful Use of the Quaternary Pump Procedure on Solvent Information Prevent Blocking of Solvent Filters Cleaning the Solvent FiltersChecking the Solvent Filters Operational Hints for the Vacuum Degasser Operational Hints for the Vacuum DegasserOperational Hints for the Multi Channel Gradient Valve Mcgv When to use the Continuous Seal Wash Option When to Use Alternative Seals Compensation Setting Optimize the CompressibilitySolvent pure Compressibility 10-6/bar Optimize the Compressibility Compensation Setting Troubleshooting and Test Functions Troubleshooting and Test Functions Status Indicators Location of Status IndicatorsPower Supply Indicator Pump Status IndicatorError Messages Timeout Shut-Down Remote Timeout Synchronization Lost Leak Leak Sensor Open Leak sensor in the quaternary pump has failed open circuitLeak Sensor Short Leak sensor in the quaternary pump has failed short circuitCompensation Sensor Open Compensation Sensor Short Fan Failed Open Cover Restart Without Cover Replace the top cover and foamZero Solvent Counter Pressure Above Upper Limit Pressure Below Lower Limit Pressure Signal Missing Missing Pressure Reading Pump Configuration Valve Fuse Inlet-Valve Fuse Valve Failed Valve 0 Failed valve aMotor-Drive Power Encoder Missing Inlet-Valve Missing Temperature Out of Range Temperature Limit Exceeded Servo Restart Failed Position for restartingPump Head Missing Broken plungerIndex Limit Index Adjustment Index Missing Stroke Length Initialization Failed Wait Timeout Pressure Test DescriptionPositioning the blank nut Pressure TestRunning the Pressure Test Running the test from the ChemStationRunning the test from the Control Module Inside the flow path definitely will cause the test to failRunning the Pressure Test Evaluating the Results If the pressure test failsPotential Cause Pump Corrective Action Potential Causes of Pressure Test FailurePotential Cause Autosampler Leak Test Half of the ramp Tools required ¼ inch wrench Parts and materials Start the test and follow the instructionsRunning the Leak Test Leak Test Pressure Profile Potential Cause Corrective Action No Pressure increase at RampPotential Cause All plateaus negative First plateau positive, second and third plateau negativeRamp 3 does not reach limit First plateau negative, second plateau positiveThird plateau negative pressure drop 2 bar/min Evaluating the Results Repairing the Pump Simple Repairs Exchanging Internal PartsCleaning the Quaternary Pump Using the ESD Strap Using the ESD StrapOverview of Repair Procedures OverviewSimple Repair Procedures Simple Repair Procedures Typical FrequencyExchanging the Active Inlet Valve Cartridge or the Active Inlet ValveRemoving the Active Inlet Valve Exchanging the Valve CartridgeReplacing the Active Inlet Valve 105 Exchanging the Outlet Ball Valve 107 Exchanging the Purge Valve Frit or Purge ValveExchanged Removing and Disassembling the Pump Head AssemblyDamage the pump drive 111 Exchanging the Pump Seals and Seal Wear-in Procedure When requiredFor the seal wear-in procedure Tools requiredWill definetely damage the normal phase application seals Seal Wear-in Procedure0905-1420 Exchanging the Pump Seals and Seal Wear-in Procedure Exchanging the Plungers PartsInstalling the Continuous Seal Wash Option 117 Lifetime of the seals Exchanging the Wash Seals 119Exchanging the Wash Seals Reassembling the Pump Head Assembly Mm hexagonal key Pump head greaseReassembling the Pump Head Assembly Exchanging the Multi-Channel Gradient ProcedureValve Mcgv 124 125 Exchanging the optional Interface Board Exchanging Internal Parts Removing the Top Cover and Foam Remove the solvent cabinet from the quaternary pump129 130 LPM Board Connectors Exchanging the Low Pressure Pump Main Board LPM BoardConnector Description McgvWA R NI N G T E Entering the Type Command Entering the Type Command using the Control ModuleEntering the Serial Number using the Control Module Entering the Type Command using the ChemStationEntering the Serial Number Entering the Serial Number using the ChemStation Replacing the Quaternary Pump’s Firmware Exchanging the Damper Disconnect the two damper capillaries Reconnect the two damper capillaries Exchanging the Fan Fan and arrow on the bottom foam142 Exchanging the Pump Drive Part required144 Reinstall the pump head and fix with the two screws Tubings and cables Exchanging the Power Supply Preparation for this procedure148 149 150 Exchanging the Power Supply Exchanging the Leak Sensor 153 Exchanging the Status Light Pipe Part requiredCover kit contains all parts, but it is not assembled Assembling the Main CoverMay not be able to remove the side from the top part Replacing the Top Cover and Foam Procedure Located in its correct placeTools required Screwdriver Pozidriv #1 Location of foam in the light switch 158 Parts and Materials Overview of Main Assemblies Repair Parts Pump Housing and Main Assemblies Front View Overview of Main AssembliesRepair Parts Pump Housing and Main Assemblies Rear View Control Module B-version Solvent Cabinet Parts Description Part Number Solvent CabinetSolvent Cabinet Parts Bottle-Head Assembly Parts Description Part Number Bottle Head AssemblyBottle-Head Assembly Parts Hydraulic Path Description Part Number Hydraulic PathHydraulic Flow Path of the Quaternary Pump Cover Parts Description Part Number Cover PartsCover Parts Sheet Metal Kit Description Part Number Sheet Metal KitSheet Metal Kit Foam Parts Description Foam PartsFoam Parts Power and Status Light Pipes Description Part Number Power and Status Light PipesPower and Status Light Pipes Leak Parts Description Part Number Leak PartsLeak Parts Pump Head Assembly Pump Head Assembly Description Part Number173 Pump Head Assembly with Seal Wash Option 175 Outlet Ball Valve Assembly Description Part Number Outlet Ball Valve AssemblyOutlet Ball Valve Assembly Purge-Valve Assembly Description Part Number Purge Valve AssemblyPurge-Valve Assembly Active Inlet Valve Assembly Active Inlet Valve Assembly Description Part NumberAccessory Kit G1311-68705 Tools and Accessories Description Part NumberSeal Wash Option Kit Seal Wash Option Description Part NumberCable Overview Cables Overview Type Description Part NumberCables AuxiliaryExternal ContactsAgilent 1100 to 3390/2/3 Integrators Analog CablesAgilent 1100 to 3394/6 Integrators Agilent 1100 to BNC Connector Agilent 1100 to General PurposeRemote Cables Agilent 1100 to 3390 IntegratorsAgilent 1100 to 3392/3 Integrators Agilent 1100 to 3394 IntegratorsAgilent 1100 to 3396A Integrators Agilent 1100 to 3396 Series II / 3395A IntegratorsAgilent 1100 to 3396 Series III / 3395B Integrators Connector Pin Active 01046-60202 Agilent TTLBCD Cables Agilent 1100 to 3396 Integrators Can Cable Auxiliary CableAgilent 1100 Series Degasser to general purposes External Contact Cable Connector Pin G1103-61611 ColorAgilent 1100 Series Interface Board to general purposes RS-232 Cable Kit Agilent 1100 module to PC RS-232 Cable Kit 34398ALAN Cables Recommended Cables196 Introduction to the Quaternary Pump Introduction to the Quaternary Pump Overview of the Quaternary Pump199 How does the Pump Work? 201 How Does Compressibility Compensation Work? Quaternary Pump DetailsHow Does Variable Stroke Volume Work? Electrical Connections 205 Instrument Layout Early Maintenance Feedback EMF EMF CountersPump Liquimeter Seal Wear CountersUsing the EMF Counters Setting the EMF LimitsElectronics Low pressure pump main board LPM, seeLow-Pressure Pump Main Board LPM Onboard Battery Block Diagram Signal ExplanationsFan Drive Electronic Fuses212 Interconnection Diagram Low Pressure Main Board LPM Resident System Firmware DescriptionMain System Firmware Updates BCD Board Optional Interface Boards2110-0004 LAN Board LAN Boards Agilent Order Number Supported networksAgilent 1100 Series Interfaces Agilent 1100 Series InterfacesAnalog Signal Output Gpib InterfaceCan Interface Regulations, see Cable Overview onAPG Remote Interface RS-232CAPG Remote Signal Distribution Pin Signal DescriptionPin Direction Function RS-232C Connection TableRS-232 Cable Setting the 8-bit Configuration Switch Bit Configuration SwitchGpib Default Addresses Communication Settings for RS-232C CommunicationDefault Addresses for Agilent Series 1100 Modules Module Address Binary AddressBaudrate Settings Forced Cold-Start Settings Stay Resident SettingsParity Settings Forced cold start has been completedStay Resident Settings Main Power Supply Assembly Main Power Supply MPS Block DiagramMain Power Supply Specifications 230 Control Module Screens for the Quaternary Pump Major keys on the Agilent 1100 Control Module Information/helpEnter MenuScreens available from the Analysis screen Setup View Pump ON/OFF Settings Screens available from the Analysis screen Fillings to their current state Analog -Status Screens available from the Analysis screen 241 Screens available from the System screen System screenSystem Screens available from the Records screen Counters after exchanging parts that are subject to wear Screens available from the Records screen 247 Maintenance logbook Screens available from the Records screen Schematics Diagnostics and Tests Tests screenPressure Test Specifications Performance Specifications Type Specification255 256 Limitation of Warranty Warranty StatementAll Chemical Analysis Products Limitation of Remedies and Liability Responsibilities of the CustomerResponsibilities of Agilent Technologies Days OnsiteGeneral Safety InformationOperation Safety Symbols Safety SymbolsSymbol Description WA R NI N G CA U T IO N Lithium Batteries Information Sound Emission Radio InterferenceManufacturer’s Declaration Flow Cell SolventsAgilent Technologies on Internet Index 268 269 270 Page This Book
Related manuals
Manual 28 pages 27.18 Kb

1100 Series specifications

Agilent Technologies 1100 Series is a highly regarded lineup of high-performance liquid chromatography (HPLC) systems designed to meet the demanding needs of laboratories in various fields including pharmaceuticals, environmental testing, and food safety. Known for its versatility, reliability, and advanced technology, the 1100 Series has established itself as a benchmark for analytical performance.

One of the standout features of the Agilent 1100 Series is its modular architecture. This design allows users to configure the system according to specific analytical requirements. The components such as pumps, detectors, and autosamplers can be mixed and matched to create a tailored solution, ensuring maximum efficiency and optimal performance. This modularity also facilitates easy upgrades and maintenance, significantly extending the life cycle of the instrument.

At the heart of the 1100 Series is its advanced pumping technology. The system utilizes precise, high-pressure pumps that ensure a consistent flow rate and excellent reproducibility, essential for accurate quantification of analytes. The binary and quaternary pump options provide flexibility in method development, enabling users to perform gradient elution with ease.

The Agilent 1100 Series also boasts a range of detectors, including UV/VIS, fluorescence, and refractive index detectors. Each detector is designed to enhance sensitivity and specificity, enabling the detection of a wide array of compounds. The UV/VIS detector, in particular, is known for its reliable performance and user-friendly interface, which simplifies method setup and data analysis.

Another critical aspect of the 1100 Series is its autosampler. The system's autosamplers provide high throughput and automatic sample handling, which significantly reduces human error and increases productivity. The intelligent design allows for temperature control and reliable sample injection, ensuring optimal results.

In terms of software, the 1100 Series is supported by Agilent’s OpenLAB software, which offers an intuitive platform for method development, data acquisition, and analysis. This software integrates seamlessly with the HPLC hardware, making it easier for scientists to manage workflows and maintain compliance with regulatory requirements.

Overall, the Agilent Technologies 1100 Series represents a significant advancement in liquid chromatography, bringing together ideal characteristics such as modularity, precision, versatility, and sophistication. Its commitment to innovation and quality makes it a preferred choice for laboratories seeking reliable analytical solutions.
Top Page Image Contents