KDa MWCO 4 mL | Agilent Technologies 5185-5991

Instructions – 4 mL Concentrators

5,000 Molecular Weight Cut-Off (MWCO)

4 mL Spin Concentrator, Part No. 5185-5991

Equipment required	4 mL Spin Concentrators, pack of 25
Centrifuge
Rotor type	Swing bucket or Fixed angle
Minimum rotor angle	25°
Rotor cavity	To fit 15 mL (17 mm) conical bottom tubes
Minimum effective speed	2,000 g
Recommended speed	5,000 g
Maximum speed	7,500 g
Concentrate recovery
Pipette type	Fixed or variable volume
Recommended tip	Thin gel loader type
Technical specifications
Concentrator sample volume capacity
Swing bucket rotor	1-4 mL
Fixed angle rotor	1-4 mL
Dimensions
Total Length	122 mm
Width	17 mm
Active Membrane Area	2.0 cm2
Hold-up volume of membrane	<10 µL
Dead stop volume	20 µL
pH range	pH 1 – 9
Materials of construction
Body	Polycarbonate
Filtrate vessel	Polypropylene
Concentrator cap	Polycarbonate
Membrane	Polyethersulfone

Concentrating Proteins with 4 mL Concentrators:		Desalting/Buffer Exchange
1	Fill concentrator with sample within the working volume	1	Concentrate sample to desired level or at least
	capacity range noted in the table above (ensure that the hinged	2	5.
2	lid is fully seated).	2	Empty filtrate container.
2	Insert assembled concentrator into centrifuge (when fixed	3	Refill concentrator with an appropriate buffer.
	angle rotors are used, angle the concentrators so that the	4	Concentrate the sample again and repeat the
3	printed windows face upwards/outwards).		process until the concentration of the
3	Centrifuge at recommended speeds, taking care not to exceed		contaminating species is sufficiently reduced.
	the maximum g force indicated in the table above (20 minutes		Typically three wash cycles will remove 99% of
	at 7,500 g is recommended for 30 concentration of 4 mL at		initial salt content.
4	10C).	See Notes on next page.
4	Once the desired concentration is achieved, remove assembly	See Notes on next page.

and recover sample from the bottom of the concentrate pocket with a pipette.