Agilent Technologies 5185-5991 Instructions - 15 mL Concentrators, mL Spin Concentrators, pack of

Instructions – 15 mL Concentrators

30,000 Molecular Weight Cut-Off (MWCO)

15 mL Spin Concentrator, Part No. 5188-2799

Equipment required	15 mL Spin Concentrators, pack of 10
Centrifuge
Rotor type	Swing bucket or Fixed angle
Minimum rotor angle	25°
Rotor cavity	To fit 30 x 114 mm conical bottom tubes or
	29 x 104 mm round bottom tubes (50mL)
Minimum effective speed	1,000 g
Recommended speed	2,000 g
Maximum speed	3,000 g
Concentrate recovery
Pipette type	Fixed or variable volume
Recommended tip	Thin gel loader type
Technical specifications
Concentrator sample volume capacity
Swing bucket rotor	2-15 mL
Fixed angle rotor	2-8 mL
Dimensions
Total Length	76 mm
Width	25.5 mm
Active Membrane Area	4 cm2
Hold-up volume of membrane	<20 µL
Dead stop volume	50 µL
pH range	pH 1 – 9
Materials of construction
Body	Polycarbonate
Filtrate vessel	Polypropylene
Concentrator cap	Polycarbonate
Membrane	Polyethersulfone

Concentrating Proteins with 15 mL Concentrators:

1Fill concentrator with sample within the working volume capacity range noted in the table above, taking care not to touch the membrane surface, and then close the hinged lid (ensure that the lid is fully seated).

2Place concentrator into 50 mL centrifuge tube (filtrate collection vessel – not supplied, user must provide).

3Insert assembled concentrator into centrifuge (when fixed angle rotors are used, angle the concentrators so that the printed windows face upwards/outwards). Spin at recommended speeds, taking care not to exceed the maximum g force indicated in the table above (20-40 minutes is common).

4Once the desired concentration is achieved, remove assembly and recover sample from the bottom of the concentrate pocket with a pipette.

Desalting/Buffer Exchange

See 4 mL Spin Concentrator instructions on the reverse side.

Notes:

￿Spin concentrators are single use disposable items. Re-use is not recommended.

￿For research use only.

￿Select the most appropriate membrane cut-off for your sample. For maximum recovery, select a MWCO at least 50% smaller than the molecular size of the species of interest.

￿These concentrators are designed for use with biological fluids or aqueous solutions only, not for organic solvents or strong acids or bases.

￿Flow rates are affected by MWCO, porosity, sample size, concentration, viscosity, centrifuge force, and temperature. Adjust centrifuge force and time accordingly for your samples.

￿Do not autoclave. Sterilize with a 70% ethanol solution.

For more information or technical assistance,	Agilent Technologies, Inc.
please call toll free: 1-800-227-9770 or visit	2850 Centerville Road
our web site at: www.agilent.com/chem/bioreagents	Wilmington, DE 19808-1610 USA

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