GE 100 reactions 79770, 188403, 33, 50 reactions 79760, 50 reactions AH9539, 500 reactions, 79750 manual

Thermo Sequenase

Radiolabeled Terminator

Cycle Sequencing Kit

Product Number 79750, 50 reactions 79760, 100 reactions 79770, 500 reactions

Product Number 188403 includes: 79750, 50 reactions AH9539, 33P-labeled

terminators

STORAGE

Store at -15°C to -30°C.

Warning: For research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.

Contents

STORAGE CONTENTS COMPONENTS OF THE KIT QUALITY CONTROL SAFETY WARNINGS AND PRECAUTIONS INTRODUCTION Chain termination sequencing Page MATERIALS NOT SUPPLIED Necessary reagents: Necessary equipment: PROTOCOL 3.Cycling termination reactions SUPPLEMENTARY INFORMATION General guidelines Preparation of template DNA Preparation of single-strandedtemplate DNA Preparation of double-strandedplasmid DNA Cycle conditions and template quantity Cycling temperatures Number of cycles and quantity of template Designing a new sequencing primer Figure Sequencing PCR Products Enzymatic pre-treatmentof PCR products Elimination of compressions Page Reading farther from or closer to the primer Denaturing gel electrophoresis Gel electrophoresis reagents Buffers Gel recipes (for 100ml of gel solution) General guidelines for electrophoresis TROUBLESHOOTING Film blank or very faint Bands faint near the primer Bands smeared Bands appear across all 4 lanes CONTROL DNA SEQUENCE REFERENCES RELATED PRODUCTS Kits and Enzymes PCR Clean-up USB Ultrapure reagents for DNA sequencing USB CORPORATION Material safety data sheet HAZARDS IDENTIFICATION FIRST-AIDMEASURES FIRE-FIGHTINGINFORMATION ACCIDENTAL RELEASE MEASURES HANDLING AND STORAGE

GE manual

Models: 100 reactions 79770 188403 33 50 reactions 79760 50 reactions AH9539 500 reactions 79750

Product Number 79750, 50 reactions 79760, 100 reactions 79770, 500 reactions

Product Number 188403 includes: 79750, 50 reactions AH9539, 33P-labeled

terminators

STORAGE