GE CONTENTS, Protocol, References, Introduction, Quality Control, Troubleshooting, Related Products, Contact Information, Control DNA Sequence

CONTENTS
Components of the Kit .......................................................................................	3
Quality Control ....................................................................................................	4
Safety Warnings and Precautions ..............................................................	4, 26
Introduction .........................................................................................................	5
Materials Not Supplied .......................................................................................	8
Protocol ...............................................................................................................	9
Supplementary Information .............................................................................	11
General guidelines .........................................................................................	11
Preparation of template DNA .........................................................................	12
Cycle conditions and template quantity .........................................................	12
Cycling temperatures .....................................................................................	12
Number of cycles and quantity of template ...................................................	13
Designing a new sequencing primer .............................................................	13
Sequencing PCR products ............................................................................	15
Elimination of compressions ..........................................................................	15
Reading farther from or closer to the primer .................................................	17
Denaturing gel electrophoresis ......................................................................	18
Troubleshooting ...............................................................................................	20
Control DNA Sequence ....................................................................................	22
References ........................................................................................................	23
Related Products ..............................................................................................	24
Contact Information .........................................................................................	25

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