Redivue nucleotides can be stored at 4°C for up to 1 week after receipt, or at a constant -20°C if longer storage is desired. Care must be taken to prevent evaporation of these small volumes of material. Tightly cap the vials after use. Store at -20°C between uses if frequency of use is less than every 1-3 days. If condensation is observed on the walls of the vial or in the cap, return the liquid to the bottom of the vial and mix well before use.

QUALITY CONTROL

All kit batches are functionally tested using 33P labeled terminators and pUC18 double-stranded DNA template as described in this protocol. Release specifications are based on sequence length, band intensity and sequence quality. The sequence must be visible up to 300 base pairs on a standardized gel with less than 24 hours exposure. The sequence must also be free of background bands strong enough to interfere with sequence interpretation.

SAFETY WARNINGS AND PRECAUTIONS

Warning: For research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.

Caution: This product is to be used with radioactive material. Please follow the manufacturer’s instructions relating to the handling, use, storage, and disposal of such materials.

Warning: Contains formamide. See ‘Material Safety Data Sheet’ on page 26.

All chemicals should be considered as potentially hazardous. We therefore recommend that this product is handled only by those persons who have been trained in laboratory techniques and that it is used in accordance with the principles of good laboratory practice. Wear suitable protective clothing such as a lab coat, safety glasses, and gloves. Care should be taken to avoid contact with skin or eyes. In the case of contact with skin or eyes, wash immediately with water (see ‘Material Safety Data Sheet’ for specific advice).

4