mediately after finishing the work, clean off the remaining oil from the lens using a soft cotton cloth wetted with xylor. Never use alcohol or immerse the top of the objective in xylor.

Be careful not to use immersion oil that has been thickened by age. The refractive index of the immersion oil should be 1.515.

7.L o n g W o r k i n g R a n g e of t h e S t a g e

The microscope L-Ke is

 

 

designed to permit large-

 

 

range lowering of its stage

 

 

(71mm)

for convenience

 

 

in observing a thick speci-

 

 

men such as for metallurgy

 

 

or culture . Unlock the lock

 

 

screw beneath the

stage

 

 

(Fig. 17), and the stage

 

 

can

be

lowered

3 3 m m

^ ^ ^ ^ ^ ^ ^

 

and

by

manipulation of

^^W^^^^^gnm^^j^^^^

'[J

the

coarse focusing knob

 

 

farther

3 8 m m .

 

 

 

The stage lowered to such a great extent may be useful in macrophoto - graphy or when a special stage is used.

8. E x c h a n g i n g t h e S t a g e

Lower the stage by means of the coarse focusing knob and then unlock the stage lock screw. The stage, then, can be removed .

MOVING THE SPECIMEN ON THE STAGE

1 . R e c t a n g u l a r M e c h a n i c a l S t a g e '*R"

This stage enables fine crosswise travelling of the slide in the range of 50 X 7 5 m m , allowing reading

of the movement down to 0.1Clip m m by the use of the vernier

provided.

For securing the slide on the stage in position, open the clip.

Each travelling is performed by rotation of two coaxial knobs iDcated one above the other on the vertical rod protruded below on the left side from the front, the upper knob being

- - 1 9 —

for longitudinal and the lower one for lateral travel of the slide on the stage.

In fluorescence microscopy or in using oil immersion ob- jectives, where the clearance between the condenser and the slide also should be oil- immersed, stiffened oil may cause unsmooth travel of the slide. In this case, removing

19of the circular opening plate at the center of the stage or fastening of the clamp screw will be helpful to a positive travel of the slide (Fig . 18).

By unlocking the lock knob on the edge of the stage the stage can be rotated horizontally for convenience in the observation from the opposite side of the microscope (Fig . 19), where the eyepiece tube is to be rotated 180°. This rotation of the stage may often be of use in photo- micrography, when the picture f o r m a t is changed from verti- cal to horizontal or vice versa. It is recommended to use the slide adapter on the stage (Fig . 20) for a sufficient longi- tudinal travel of the slide in such reversed position.

2 . C i r c u l a r F l o a t i n g S t a g e

The circular floating stage (Fig . 21) glides and rotates smoothly and precisely in any desired direction simply by pushing the rim of the stage by the fingers, wichin a circle of 1 8 m m in diameter .

To fasten the floating stage in position, pressing the stage

Page 11
Image 11
Nikon L-Ke manual Moving the Specimen on the Stage, O n g W o r k i n g R a n g e of t h e S t a g e