OPERATION OF THE PHILIPS CM-200 FEG-TEM

When not in use, the CM-200 should be in the MICROSCOPE ON configuration with the HIGH TENSION ON (illuminates green when the high tension is on).

. The microscope is normally never turned off.

Preliminary

•Add liquid Nitrogen to the Dewar next to the sample holder. (Anticontamination device:) Place the metal-clad glass vacuum dewar into its holder to the right of the column so that the soft copper wire "beard" hangs inside it. The glass viewing windows should be covered for safety. Fill the dewar nearly full with liquid nitrogen and place the styrofoam cap on top.

•Log in (in the hallway)

•Go to vacuum page by pressing button Ready then button Vacuum. Make sure pressure reading of IGP is below 10, preferably at 5 before proceeding to the next step.

1.Specimen Holder Removal, Loading, and Insertion

Never remove or insert the specimen holder when the red indicator light (on the front of the compustage housing) is on. Do not touch the leading edge of the specimen holder (from the o-ring to the tip) with ungloved hands. This portion resides inside the vacuum and must be kept clean.

There are several types of sample holders. The first type is single tilt (tilt in X or Alpha direction). The second type is double tilt (tilt in both X and Y directions). Cold holder consists of a sample holder and a chamber to contain liquid nitrogen. Thermometer and heating systems are attached. Hearing holder can be used to hold samples that needed to be heated (up to 700°C) during microstructure exploring.

1.1REMOVAL: To remove the specimen holder from the column, carefully pull the round black handle straight out until it stops, and hold it firmly so that it does not get pulled back into the 'scope by the vacuum. Now rotate it clockwise until it stops again; it may now be pulled straight out (carefully), free of the column. The specimen holder should be set down only on its Lucite stand.

1.2SPECIMEN LOADING:

(i)Use the pin tool (located in the Lucite stand, under the tip) to lift the grid clamping device at the tip of the specimen holder).

(ii)Transfer your grid to the specimen holder using forceps. To make scanning the grid easier, you may wish to orient one set of grid bars parallel to the long axis of the specimen holder.

(iii)Use the pin tool to carefully lower the clamping device onto the grid and lock it in place.

1.3 INSERTING THE SPECIMEN HOLDER INTO THE COMPUSTAGE:

(i)Touch the sample holder tip to one of the aperture handle to discharge the sample holder.

(ii)With the small pin in the holder tip at the 11:00 o'clock position, carefully insert the specimen holder into the airlock entryway at the center of the compustage. Insertion of the holder will initiate the pre-pumping sequence, and the red indicator light on the front of the compustage will come on. Slide the holder in until it stops; at this point it will not go all the way in. (The data

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Philips CM-200 manual Specimen Holder Removal, Loading, and Insertion, Specimen Loading
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CM-200 specifications

The Philips CM-200 is a sophisticated electron microscope designed to facilitate high-resolution imaging and analysis of materials at the nanoscale. Renowned for its robust performance and versatility, the CM-200 is utilized extensively in both research and industrial applications, particularly in materials science, biology, and nanotechnology.

One of the key features of the CM-200 is its advanced electron optics, which utilizes a high-tension generator to produce a stable and finely focused electron beam. This enables researchers to achieve magnifications of up to 1,000,000 times, allowing for the visualization of structures that are mere nanometers in size. The high resolution is further complemented by a large depth of field, which provides unprecedented clarity and detail in imaging complex samples.

The CM-200 also incorporates a range of imaging modes, including bright field, dark field, and selected area diffraction imaging. These modes allow for a comprehensive analysis of the sample under various conditions, making it easier to identify structural features and materials' properties. Furthermore, the microscope is equipped with a state-of-the-art camera system, which captures high-quality images and facilitates real-time observation.

In terms of sample preparation, the CM-200 supports various techniques, including ultra-thin sectioning and cryo-preparation methods. This flexibility makes it suitable for analyzing a wide array of sample types, from biological tissues to advanced material composites. The ability to handle both conductive and non-conductive samples without significant alteration ensures that the integrity of the sample is maintained throughout the imaging process.

Moreover, the CM-200 is designed with ease of use in mind. Its intuitive interface and automated functions streamline operation, allowing users to focus on analysis rather than technical adjustments. This user-friendly design promotes efficiency and accessibility for both novice and experienced researchers.

In summary, the Philips CM-200 is a cutting-edge electron microscope that combines advanced technologies with user-centric features. Its high-resolution imaging capabilities, versatile imaging modes, and adaptability for various sample types make it an invaluable tool in scientific research and industrial applications. Researchers can rely on the CM-200 to provide detailed insights into the nanoscale world, driving advancements across multiple fields.
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