monitor screen will automatically open to the HOLDER SELECTION PAGE. Press the appropriate key (in most cases you'll choose NO COMPUSTAGE B TILT) and then press the READY button (below the data monitor screen). The data monitor screen will return to the previously selected page (normally TEM BRIGHTFIELD).)

(iii)After the red indicator light goes out, grip the specimen holder firmly by its black plastic handle and rotate it counterclockwise to 6:00 o'clock position. When it reaches the limit of its rotation, the vacuum will begin to pull the holder deeper into the column. Maintain a firm grip on the handle as the holder enters the column so that it does not get drawn in too quickly. Once the holder appears to be all the way in, jiggle it very gently to ensure that it is indeed inserted completely into the column.

2.Initiating Operation

2.1When the CM-200 is ON, the white STAND BY and red MICROSCOPE OFF buttons will be illuminated; the ON button will be dark. The lit buttons indicate their availability as emergency functions while the microscope is running.

2.2Depress the PANEL DIM knob to illuminate the data monitor screen and emission gauge. Clockwise rotation of this knob increases the intensity of the panel lighting and the light on the flexible stalk. Clockwise rotation of the DATA DIM knob increases the brightness of the data monitor screen.

2.3Ensure that the UHV and HIVAC indicator lights are lit (green). If they are not it may be necessary to press the VACUUM SYSTEM ON button and wait (20 to 30 minutes) until the two lights come on, indicating that operational vacuum status has been attained.

2.4The data monitor screen will display the CM-200 PHILIPS MICROSCOPE STATUS startup page or, more likely, one of the MODES or MODE SELECTION pages. If the READY light (on the pushbutton directly below the data monitor screen; to the right) is lit, pressing it will move the screen selection back in the hierarchy of pages. Press the MODES key to obtain the MODE SELECTION page.

2.5For transmission electron microscopy, press the HR-TEM key on the MODE SELECTION page. If the letters HR-TEM are highlighted it will be necessary to press only once; otherwise press the HR- TEM key twice. The screen should now display the HR-TEM BRIGHTFIELD page.

2.6Press the VACUUM key to check the vacuum status of the instrument. It should read READY at the top center of the data monitor screen. If it reads START-UP the operator must wait for the vacuum to improve before the 'scope can be used. Do not uses the microscope unless the ion getter pump (IGP) reading is lower than 10.

2.7To return to the TEM BRIGHTFIELD page press the READY button.

2.8To select the operating voltage, press the PARAMETERS key to open the PARAMETERS pages. On the first of these pages the kV may be modified by pressing the left (lower) or right (higher) key adjacent to the HIGH TENSION kV notation on the screen. Most TEM users in this laboratory work at 200 kV.

2.9Press Ready, Mode, choose configuration page. Slowly increase the filament voltage to 3.8. As a rule of thumb, rotate the knob until you hear three clicks; wait 20 seconds; rotate again for three clicks; wait another 20 seconds; repeat until reaching 3.8.

2.10Open the valve (from Close to Open, unti-clockwise) to the right of the microscope. You should see the beam. Move sample feature to the middle of the viewing area.

2.11Also on the first PARAMETERS page is the EMISSION setting, which should be left at 3. Press the READY button to return to the TEM BRIGHTFIELD page.

2.12With the high tension on, an operator may load his/her own previously stored or default alignment/stigmator settings for the kV at which the 'scope is operating. This requires logging into the computer (rodin) on the table to the right of the microscope, obtaining a CRT terminal screen, and typing, e.g., 's_toscope default' to load the default settings for the currently selected kV. A separate laminated sheet of instructions in the TEM room fully describes these functions.

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Philips CM-200 manual Initiating Operation

CM-200 specifications

The Philips CM-200 is a sophisticated electron microscope designed to facilitate high-resolution imaging and analysis of materials at the nanoscale. Renowned for its robust performance and versatility, the CM-200 is utilized extensively in both research and industrial applications, particularly in materials science, biology, and nanotechnology.

One of the key features of the CM-200 is its advanced electron optics, which utilizes a high-tension generator to produce a stable and finely focused electron beam. This enables researchers to achieve magnifications of up to 1,000,000 times, allowing for the visualization of structures that are mere nanometers in size. The high resolution is further complemented by a large depth of field, which provides unprecedented clarity and detail in imaging complex samples.

The CM-200 also incorporates a range of imaging modes, including bright field, dark field, and selected area diffraction imaging. These modes allow for a comprehensive analysis of the sample under various conditions, making it easier to identify structural features and materials' properties. Furthermore, the microscope is equipped with a state-of-the-art camera system, which captures high-quality images and facilitates real-time observation.

In terms of sample preparation, the CM-200 supports various techniques, including ultra-thin sectioning and cryo-preparation methods. This flexibility makes it suitable for analyzing a wide array of sample types, from biological tissues to advanced material composites. The ability to handle both conductive and non-conductive samples without significant alteration ensures that the integrity of the sample is maintained throughout the imaging process.

Moreover, the CM-200 is designed with ease of use in mind. Its intuitive interface and automated functions streamline operation, allowing users to focus on analysis rather than technical adjustments. This user-friendly design promotes efficiency and accessibility for both novice and experienced researchers.

In summary, the Philips CM-200 is a cutting-edge electron microscope that combines advanced technologies with user-centric features. Its high-resolution imaging capabilities, versatile imaging modes, and adaptability for various sample types make it an invaluable tool in scientific research and industrial applications. Researchers can rely on the CM-200 to provide detailed insights into the nanoscale world, driving advancements across multiple fields.