As the focus is adjusted on a view camera, the image also changes size, and therefore moves slightly as a function of its distance from the optical axis. The human
C. Focus display for a poorly chosen focus location without distinct edges.
Both of these problems may be mitigated by using the Magnification slider in the Focus tab panel to change the system’s effective magnification and viewing area. Increase the magnification and the sampling area will decrease; decreasing the magnification has the opposite effect. A higher magnification allows finer details to be resolved better, and also shrinks the size of the viewing rect- angle to align better with small objects in the set. A lower magnification covers a longer section of the image, providing more data in
Reading the Focus Displays and Adjusting Focus
After the CCD is moved to the desired focusing location, readout and display of the selected channel’s image data begins. The number of pixels output and displayed is fixed at 100; the various available magnifications utilize different resolution settings to achieve different spans of coverage. The largest sampling area, 4X magnification, uses one of the lowest resolution settings, which combines many CCD pixels into each output pixel. The highest magnification and smallest sampling area, 32X, uses one of the highest resolution settings, so few CCD pixels are combined into each output pixel. The CCD is not moving during the focus operation, instead, the same portion of the selected channel is read out and displayed as often as possible, usually faster than ten updates per second.
As each line segment is read out, the selected pixels are first displayed as a graph of brightness vs. position in the rectangular box on the right side of the Focus panel. This graph shows each output pix- el’s
This line graph can be helpful in establishing approximate focus, and in verifying that the desired image features are indeed being viewed (by correlating relative brightness and tex- ture, etc.), and have adequate variations for measuring focus.
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