Chapter 6: Maintaining Your Filtered Enclosure

Annually

Replace the fluorescent lamps. Replace UV lamps if equipped.

Have the enclosure validated by a qualified certification technician. See Certification and Recertification in Chapter 6.

All monthly activities.

Decontamination

When used in conjunction with biohazards, the Purifier Class I Enclosure and Purifier HEPA Filtered Enclosure should be decontaminated with formaldehyde gas before:

maintenance work in contaminated areas

HEPA filter changes

moving the cabinet to a new location

changing research programs

after a gross spill of biohazardous material

The procedures for performing a gaseous decontamination are thoroughly outlined in the U.S. Department of Health, Education and Welfare booklet entitled Formaldehyde Decontamination of Laminar Flow Biological Safety Cabinets, available from NIH, Division of Safety, Bethesda, MD 20892, call 301-496-2801, or from Labconco Corporation.

Determination of when to Replace

HEPA Filters

The HEPA filters in the filtered enclosures gradually accumulate airborne particulate matter and powders from the enclosure and room. The rate of accumulation will depend upon the cleanliness of the room air, the amount of time the enclosure is operating, and the nature of work being done in the enclosure. In typical installations and usage, the HEPA filters will last two to five years before requiring replacement. Replace HEPA filters when face velocity drops below the recommended 75-105 fpm velocity, and the speed control is adjusted to full speed. Replace HEPA filters if it fails the HEPA Filter Leak Test in Chapter 6.

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Gentek 3980303, 3980423, 3980422, 3980200, 3980401 Decontamination, Determination of when to Replace Hepa Filters, Annually

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