8.Diaphragm: The Diaphragm (9) is placed in the light path to alter the amount of light that reaches the condenser within the Microscope (for enhancing contrast in the image). (See Assy. Diagram.)
9.Base: The Base (12) supports the weight of all of the Microscope parts.
(See Assy. Diagram.)
Specimen Preparation:
1.When observing a specimen by transmitted light, light must pass through the specimen in order to form an image. The thicker the specimen, the less light passes through. The less light that passes through, the darker the image.
2.The specimens must be thin (0.1 to 0.5mm).
3.Many specimens must be cut into thin sections before observation. Specimens such as rock or semiconductors are too thick to be sectioned and observed by transmitted light, so they are observed by the light reflected from their surfaces.
MicroscopicTerms:
1.Depth of Field: The vertical distance, from above to below the focal plane, that yields an acceptable image.
2.Field of View: The area of the specimen that can be seen through the Micro- scope with a given Objective Lens (5).
3.Focal Length: The distance required for an Objective Lens (5) to bring the light to a focus (usually measured in microns).
4.Focal Point/Focus: The point at which the light from an Objective Lens (5) comes together.
5.Magnification: The product of the magnifying powers of the Objective Lenses
(5) and Zoom Eyepiece (1). The numbers marked on the Objective Lenses indicates how many times the specimen on the Slide (7) is being magnified. The Objective Lenses on this Microscope allow a specimen to be magnified from eight (8X) to twenty (20X) times its actual size.
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