(2)Hold the specimen holder with the
(3)After hearing a click sound, grape the specimen holder and turn clockwise, the specimen holder will slide into a groove in the airlock by vacuum.
(4)Wait until the airlock indicator light extinguishes, then rotate the holder counter clockwise until it reaches a position where it will slide inwards. The holder is pulled strongly inward by the pressure differential, which may damage the sapphire pivot at the holder tip by colliding with the thrust rod of the specimen control. We recommend to keep your hand on the specimen holder handle and keep it moving slowly inward.
(5)Turn counterclockwise a few degrees and insert the specimen holder to the beam path (C position).
IIIRoutine Alignment (See Alignment Procedures for CM-20 TEM)
IV. Shut Down
(1)Set Magnification at 100 kx.
(2)Remove objective aperture and SAD apertures (levers to the right).
(3)Press MODES on TEM bright field page.
(4)Press CONFIGURATION on mode selection page.
(5)Turn the FILAMENT knob counterclockwise slowly (with
(6)Set sample stage to center position (right = 0, left = 10).
(7)Make sure goniometer is locked and back to 0 tilt position (tilt = 0, azimuth = 11).
(8)Carefully remove specimen holder out of the microscope column.
(9)Remove your specimen and insert the specimen holder into the microscope (keep in high vacuum).
(10)Cover viewing ports.
(A) If no pictures taken:
(1)Press READY twice to go back
(2)Pull the PANEL DIM knob and leave the microscope for the next user (do not turn off the high voltage).
(3)Fill out TEM log book.
(4)Clean the desk and keep the microscope room clean.
(B) If Pictures taken:
(1)Press VACUUM on TEM bright field page.
(2)Press CAM AIR on vacuum status page to leak air (nitrogen) into the camera.
(3)Wait for 3 minutes and then replace the camera box in the microscope with the camera box in the
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