19
Protocol
9. Stop the reaction by the
addition of 5 µl of 0.2 M
EDTA. For use in a
hybridization, denature the
labelled DNA by heating to
95-100°C for 5 minutes, then
chill on ice continued.
Notes
9. Continued
described in Appendix III.
Calculation of probe specific
activity is described in
Appendix II.
Extensive experimentation
with Rapid-hyb buffer
(RPN1635/6) has shown that
probe purification, even
under the conditions given
above is not required with
the isotopes
32P and 32P.
Purification of
32S labelled
probes is however required
to reduce filter background.