GE RPN1605, RPN1606, RPN1607, RPN160A

using 17 pmol/25 ng DNA of

[α–32P] labelled nucleotides,

specific activity 3000 Ci/mmol

(codes PB 10204-7) and

RPN 1606/1607 are tested

using 17 pmol/25 ng DNA of

[α–32P]dCTP, 3000 Ci/mmol

(code PB 10205). Incorporations

greater than 55% are achieved

after 10 minutes incubation

at 37°C, as assayed by thin-

layer chromatography on PEI

cellulose in 1.25 M KH2PO4.

PH3.4.

In addition components of the

kits are checked for identity by

HPLC and the DNA solutions

for concentration by UV

spectrophotometry.

Contents

Main Page finder 1. Legal 2. Handling 2.1. Safety warnings and precautions 2.2. Storage and stability 2.3. Quality control Page 3. System components Page 3.1. Megaprime DNA labelling systems 4. Introduction Page 5. Megaprime DNA labelling protocols 5.1. Standard Megaprime protocol Page Page Page 5.2. New Megaprime protocol Page Page Page Page 5.3. Use of alternative reaction conditions Page } } Page Page Page Page 6. Appendices 6.1. Appendix I. Labelling of DNA fragments in low melting point agarose Page Page Page Page 6.3. Appendix III. Removal of unincorporated nucleotides Page 6.4. Appendix IV. Additional equipment and reagents 7. Troubleshooting guide Page Page 8. References 9. Related Products Page Page imagination at work RPN1604PL Rev B 2006 GE Healthcare regional office contact numbers: GE Healthcare offices: