b) Incorporation efficiency
i) One labelled dNTP
ii) Two labelled dNTP
iii) Three labelled dNTP
Figure 3. The use of [α–32P]dNTPs in the Megaprime DNA labelling
system (see notes on page 26).
24
Percentage of added label
100
80
60
40
20
(i)
(ii)
(iii)
0 10 20 30 40 50 60 70 80 90 100
Total input label (pmols)