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Problem
1. Low signal
Possible cause
1. Incomplete
denaturation of
template DNA
2. Low probe
concentration
3. Low probe specific
activity
Remedy
1. Ensure denaturation
protocol is followed.
2. Accurately measure
the concentration of
template DNA used in
the labelling reactions.
Check recovery of
probe if purification
is performed to
remove unincorporated
nucleotide.
3. If the specific activity
of the labelled DNA is
lower than expected,
a labelling reaction
should be carried out
using a sample of the
control DNA supplied
with the system.
If this proceeds
satisfactorily, check
the concentration
and purity of your
DNA.
7. Troubleshooting guide
If poor results are obtained, the following guide may help to
determine the cause of the problem.