1 GENERAL USER INFORMATION

1

2 SETTINGS FOR TRANSMITTED LIGHT MICROSCOPY

1

2.1

Turning on the microscope

1

2.2

XY-stage

1

2.3

Placing the sample

2

2.4

Objectives

2

2.5

Focusing

3

2.6

Eye pieces (oculars)

4

2.7

Dual viewing attachment

4

2.8

Koehler illumination

6

2.9

Polarization contrast

8

2.10

Filters

8

2.11

Dark-field microscopy

9

2.12

Turning off the microscope

9

3

IMAGE ACQUISITION

10

 

3.1

Accessing the computer

10

 

3.2

View Finder application

10

 

3.3

Adjustments before image acquisition

10

 

3.3.1

Capture resolution

10

 

3.3.2

Sensitivity

10

 

3.3.3

Exposure Mode

11

 

3.3.4

White balance

11

 

3.3.5

Levels adjustment

11

 

3.3.6

Focus tool

12

 

3.3.7

Over/Underexposure

12

 

3.3.8

Averaging

12

 

3.4

Capturing the image

13

 

3.5

Viewing images

13

 

3.6

Post-imaging adjustments

13

 

3.6.1

Cropping the image

13

 

3.6.2

Scale bars

14

4 SAVING AND TRANSFERRING IMAGES

14

5

FINISHING YOUR IMAGING

14

6

TROUBLESHOOTING

15

 

6.1

There is no light

15

 

6.2

Light is too dark

15

 

6.3

Problems with focus or picture clarity

15

 

6.4

View is partial

15

 

6.5

Colors have red/yellow tint

15

 

6.6

Ocular view is not at the same focus as the camera view

15

 

6.7

Computer login fails

16

Page 2
Image 2
Leica B523 manual Saving and Transferring Images