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Leica
B523 manual
1
1
18
18
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18 pages, 1.59 Mb
Leica DB LB Research microscope
and Studo Lite Imaging software
Room B523
User Guide
Molecular Imaging Unit
University of Helsinki
www.miu.helsinki.fi
9.4.2008
Contents
Main
Leica DB LB Research microscope and Studo Lite Imaging software
Page
1 General user information
2 Settings for transmitted light microscopy
2.1 Turning on the microscope
2.2 XY-stage
2.3 Placing the sample
2.4 Objectives
2.5 Focusing
2.6 Eye pieces (oculars)
2.7 Dual viewing attachment
Page
2.8 Koehler illumination
Page
2.9 Polarization contrast
2.10 Filters
2.11 Dark-field microscopy
2.12 Turning off the microscope
3 Image acquisition
3.1 Accessing the computer
3.2 View Finder application
3.3 Adjustments before image acquisition
Page
Page
3.4 Capturing the image
3.5 Viewing images
3.6 Post-imaging adjustments
4 Saving and transferring images
5 Finishing your imaging
6 Troubleshooting
6.1 There is no light
6.2 Light is too dark
6.3 Problems with focus or picture clarity
6.4 View is partial