3. Installation
3.2.Tubing connec- tions
3.3Recom- mended bath liquids
3.4Filling and draining the bath
Silicone tubing (inner diameter 8 mm) can be used over the entire working temperature range of the unit. PVC tubing can only be used in the temperature range of 10 to 60 °C.
1.Connect the inlet and outlet tubing to the nozzles on the rear of the circulator (Fig. 1).
2.Connect the other ends to the electrophoresis unit. The nozzles have an outer diameter of 9.5 mm. To ensure safe tubing connections, secure all tubing with hose clamps.
When MultiTemp III is used as a water bath, the nozzles should be connected to each other.
Condensation of water on cold tubing can be avoided by covering it with insulating tubing. (See Insulation for cooling tubing in the Ordering Information section.)
Condensation on the cooling plate of Multiphor II during sample application can be avoided by shutting off the circula- tion to the cooling plate with a three way valve. (See
Note: Do not touch or remove tubing when the unit is in operation. Stay alert to the dangers of hot water. The surface temperature of the tubing can reach 70 °C at high working temperatures.
Use distilled water in the temperature range of 5 to 90 °C. A mixture of distilled water and ethylene glycol in a concentra- tion up to 20% is suitable for lower ranges
Warning! Ethylene glycol is poisonous. Handle with care and follow recommended safety procedures.
Warning! Methanol and ethanol are not recommended becau- se of their low flash points. Both liquids are unsafe to use.
Remove the plastic lid and fill the bath with liquid. The dis- tance between the surface of the liquid and the stainless steel top plate should be about 2 cm. If it is more than 4 cm, a sensor will activate the temperature limit switch and cause
To drain the bath, switch off the mains power and remove the outlet tubing from the external equipment and let the
7