Agilent Technologies G1978B setup guide 20 1 pk-pk 10 1 pk-pk

Verification 2

To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive SIM Modes

Table 2 6130 and 6140 Quad Performance Verification Summary, SIM Mode

	G1978B	G1978B
	ES Positive SIM Mode	APCI Positive SIM Mode
Solvent	75:25	75:25
	methanol/water	methanol/water
	with 5mM	with 5mM
	ammonium formate	ammonium formate
Method name	6130SIMES_MM.M	6130SIMCI_MM.M
	(6130 model)	(6140 model)
	6140SIMES_MM.M	6140SIMCI_MM.M
	(6140 model)	(6140 model)
Performance Specification	20: 1 pk-pk	10: 1 pk-pk
	100: 1 rms	50: 1 rms

Sensitivity Verification for Multimode in ESI, Positive SIM Mode

1Start up the ChemStation software.

2Change the view to MSD Tune, and start an Autotune.

After the autotune completes, you may need to wait up to 30 minutes to allow for the calibrant solution to be pumped out of the Quad instrument. This minimizes any background signal resulting from the calibrant.

3Change the view to Method and Run Control, and load the method 6130SIMES_MM.M (6130 model) or 6140SIMES_MM.M (6140 model).

4Edit the method to ensure that 75:25 methanol/water with 5mM ammonium formate is selected as the LC solvent.

5Place the vials into the LC autosampler.

•Position #1: empty, uncapped vial

•Position #2: vial of the solvent used for dilution (solvent blank)

•Position #3: vial with the reserpine sample (1 pg/µL)

6Run the method.

The method does an FIA run with one injection of the empty vial, five injections of the solvent blank, and five injections of the reserpine sample.

You may need to further optimize the nebulizer pressure by running FIA

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 25