2Verification

To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive SIM Modes

experiments to achieve maximum instrument sensitivity

7Review the results.

When the method is finished, a report prints showing the signal-to-noise ratio for the five blank and five sample peaks, and a blank-subtracted average of the sample peaks. The blank-subtracted average signal-to-noise value must be greater than or equal to 20:1 peak-to-peak (100:1 RMS).

Sensitivity Verification for Multimode in APCI, Positive SIM Mode

1Start the ChemStation software.

2Change the view to MSD Tune, and start an Autotune.

After the autotune completes, you may need to wait up to 30 minutes before you continue, to allow for the calibrant solution to be pumped out of the Quad instrument. This minimizes any background signal resulting from the calibrant.

3Change the view to Method and Run Control, and load the method 6130SIMCI_MM.M (6130 model) or 6140SIMCI_MM.M (6140 model)

4Edit the method to ensure that 75:25 methanol/water with 5mM ammonium formate is selected as the LC solvent.

5Place the vials into the LC autosampler.

•Position #1: empty, uncapped vial

•Position #2: vial of the solvent used for dilution (solvent blank)

•Position #3: vial with the reserpine sample (1 pg/µL)

6Run the method.

You may need to further optimize the nebulizer pressure by running FIA experiments to achieve maximum instrument sensitivity

The method does an FIA run with one injection of the empty vial, five injections of the solvent blank, and five injections of the reserpine sample.

7Review the results.

When the method is finished, a report prints showing the signal-to-noise ratio for the five blank and five sample peaks, and a blank-subtracted average of the sample peaks. The blank-subtracted average signal-to-noise value must be greater than or equal to 10:1 peak-to-peak (50:1 RMS).

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Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide

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Agilent Technologies G1978B setup guide Verification
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G1978B specifications

Agilent Technologies G1978B is a high-performance mass spectrometer that is widely recognized for its innovations in analytical chemistry. This instrument stands out due to its combination of advanced technologies that enhance sensitivity, accuracy, and precision for complex analyses.

One of the key features of the G1978B is its compatibility with various ionization techniques, including Electrospray Ionization (ESI) and Atmospheric Pressure Chemical Ionization (APCI). This versatility allows users to analyze a diverse range of sample types, from small molecules to large biomolecules. Each ionization technique has its unique advantages, making the G1978B an excellent choice for laboratories focusing on pharmaceuticals, environmental analysis, and proteomics.

The G1978B is equipped with a sophisticated quadrupole mass filter, ensuring high-resolution mass measurements. This technology provides excellent mass accuracy and reproducibility, enabling users to distinguish between closely related compounds with minimal interference. The advanced data acquisition systems incorporated in the G1978B allow for rapid scanning capabilities, making it particularly effective for high-throughput screening applications.

In addition, the instrument features an intuitive user interface that simplifies method development and operational tasks. This includes streamlined software solutions that facilitate data analysis and interpretation, ensuring that even complex datasets can be managed efficiently. Furthermore, built-in diagnostic tools help in monitoring instrument performance, significantly reducing downtime and enhancing productivity.

The G1978B also incorporates advanced technologies for enhanced sensitivity. Its innovative source design minimizes background noise while maximizing signal intensity, making it particularly valuable for the detection of trace-level contaminants or low-abundance metabolites. Coupled with its exceptional dynamic range, the G1978B can quantify analytes over several orders of magnitude, which is crucial for applications in toxicology and clinical research.

With a robust design and ease of integration into existing laboratory workflows, the Agilent G1978B provides an ideal solution for researchers seeking a reliable mass spectrometry platform. Its combination of advanced ionization techniques, high-resolution capabilities, and user-friendly software makes it a versatile tool in modern analytical laboratories, paving the way for breakthroughs in research and development across a multitude of scientific disciplines.
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