Agilent Technologies G1978B setup guide Quad Performance Verification Summary, Scan Mode

Verification 2

To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes

To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes

Preparation

1Transfer 1 mL of 5 ng/µL reserpine (Agilent G2423A) to a 50 mL volumetric flask. Use a clean graduated pipette.

2Dilute to the 50 mL mark with 75:25 methanol/water with 5 mM ammonium formate.

3Transfer 5 mL of the first dilution to a 50 mL volumetric flask. Use a clean graduated pipette.

4Dilute to the 50 mL mark with 75:25 methanol/water with 5 mM ammonium formate.

This provides the final 10 pg/µL reserpine concentration required for performance verification.

5Transfer approximately 1 mL of the second dilution to an autosampler vial.

Table 3	6130 and 6140 Quad Performance Verification Summary, Scan Mode
		G1978B	G1978B
		ES Positive Scan Mode	APCI Positive Scan Mode
Sample		Reserpine, 5 ng/µL	Reserpine, 5 ng/µL
Concentration after dilution		10 pg/µL	10 pg/µL
Injection volume		5 µL	5 µL
Total sample amount injected		50 pg	50 pg
Sample order number		G2423A	G2423A
Solvent		75:25	75:25
		methanol/water	methanol/water
		with 5mM	with 5mM
		ammonium formate	ammonium formate

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 27