Olympus FV300 Colocalization, Link with patch clamping data, Threshold Mode, Regions/Min-Max Mode

Colocalization

Analyzing the degree of intensity overlap between channels.

Thresholds Mode	Regions/
	Min-Max Bound Mode

Colocalization

By using this function to analyze multi-color specimens, it is possible to discover whether different labeled substances are present in the same region. The ability to quantify the Pearson correlation, the overlapping coefficient and the colocalization index allows colocalization volumes to be compared between different specimens. Images can also be analyzed in series.

Colocalization image (white)

Threshold Mode

Threshold lines can be interactively altered.

Regions/Min-Max Mode

Setting the ROI (region of interest) on the histogram makes it possible to create a colocalization image. Values can also be obtained for Pearson correlation, overlapping coefficient and colocalization index.

Physiolink

Analyzing the state of a cell interior by synchronizing electrophysiological and confocal

image data. (Optional software).

Physiolink software (FV300 side)

Physioview software (Axon PC side)

Working conditions: Clampex Ver. 8.0 or later

Pacemaker neuron: Sea-slug (nudibranch)

Dr. Stuart Thompson, Department of Biological Sciences, Hopkins Marine Station, Stanford University.

Link with patch clamping data

•With PCs linked through a LAN, Physiolink can synchronize electrophysiological and confocal image data simultaneously.

•With the same time stamp recorded in the two PCs, it is possible to access and analyze an image and its patch clamping data corresponding to the Physiolink software time scale.

•The patch clamping graph and Physiolink software are interlocked and activated concurrently.

•Physiolink software complies with the FV300’s high-speed scanning, enabling msec analysis.

Ethernet

FV300	Axon
	Clampex

Connecting two PCs by LAN is required.

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