Olympus FV300 manual Easy exchange between display methods, Image tool bar, Thumbnail display

Easy exchange between display methods

Independent navigation bars for each image window enable the display method to be changed quickly and easily.

Image tool bar

X-Y-Z scanning operations and time- lapse observations both produce multiple images, which can be displayed in sequence simply by clicking the sequential mode button. Channel selection and image zooming are also available on the same menu.

Thumbnail display

Data stored in the gallery window are displayed as thumbnails for easy viewing.

Tiling display function for see-at-a- glance comparison of multiple images

The FV300's live tiling function, which is especially valuable in time course experiments, allows observations of multiple images or changes in the specimen while the experiment is in progress. Images in series (e.g. XYt or XYZ) can be freely displayed.

Scanning unit set-up monitor display

Single monitor display is also possible.

Sequential scanning to prevent cross talk

Sequential scanning may be used to minimize the fluorescence cross talk often seen between channels in multicolored samples. This is achieved by exciting each fluorochrome independently, one dye at a time. With the AOTF function, line sequential scanning is available as well.

* Once optimized: steps q-r can be performed easily

q Excited by only HeNe633 w Excited by only Kr568 e Excited by only Ar488

rComposition

Human colon crypt

Nuclei (Blue) TO-PRO-3

Actin (Green) Alexa 488

APC gene product (Red) Alexa 568

Christine Anderson,

Laboratory of Prof. Ray White,

Hunstsman Cancer Institute, Utah

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