Software Graphical User Interface

Ultimate ease of operation and monitor display.

Dye selection display

When a fluorescence dye is chosen, the laser and light path settings are selected automatically, with each of the selected fluorescence dyes displayed graphically on the monitor.

Versatile display options

Exchange between condensed and full image display modes can be performed with a single touch. Individual panel layouts can be changed at will, and the panel in use can be placed in any desired position.

Simplified toolbar

A newly designed toolbar with various dedicated buttons has greatly improved ease of use. The user can execute a succession of selected processes with one-click operation for each.

Preset the conditions for image acquisition and loading

Storage of Acquisition Settings enables immediate, one-touch recall of all the relevant experimental settings and conditions. Adding new conditions or altering existing ones is quick and easy.

Innovative scanning method for improved performance

 

ZoomIn scanning

 

Zoom scanning can be

 

conducted over any

ZoomIn

designated rectangular area.

Since only the region of the

 

targeted, zoomed-in area is acquired, scan time and laser exposure of the specimen is minimized.

 

Point scanning

 

The ultimate in fast scanning,

 

the point scan enables

Spot

accurate quantitation of

intensity changes during rapid

 

 

physiological events.

Line scanning

A single line may be scanned, oriented at any angle in the XY

SlantLine plane. This fast scanning option permits accurate

quantitation of physiological events such as Calcium waves or sparks.

Flexible setting of scanning size, zoom, movement and rotation

The observation field and scanning area are both displayed graphically. Settings can be confirmed while scrolling through the zoom ratios. The “pan” button lets the operator move the image acquisition area at will, and rotation scanning of images is also possible.

Free line scanning

Intensity changes may be measured over a given period

FreeLine of time along the length of a freely drawn line, such as the

trace of an axon or along a cellular junction.

Clip scanning

By cropping the image, selected areas can be cut out of complex image stacks.

AOTF: flexible control of the laser intensity to meet the specific demands (optional)

The laser exposure will be limited within the scanning area by default, minimizing unnecessary bleaching of the specimen. Option includes:

•Any laser intensity for any excitation area ("Region of Excitation" mode)

•Multiple laser applications

•AOTF controller that provides easy link with external equipment

20%

50%

B

3

Page 4
Image 4
Olympus FV300 manual Software Graphical User Interface