Olympus FV300 manual Specifications

Specifications

	Item	Specifications
Laser light	Visible light laser source	Select from the following laser, to mounted on laser combiner
source		Multi-line Ar laser (458nm, 488nm, 515nm, Total 40mW), Ar laser (488nm,10mW), Kr laser (568nm, 10mW),
		HeNe (G) laser (543nm,1mW), HeNe (R) laser (633nm,10mW), LD405 (405nm, 25mW), LD440 (440nm, 5.3mW)
	Laser combiner	Each laser light path is equipped with a continuously variable neutral density filter or AOTF
		All laser lines are combined to apsis along the same fiber optic
Scanning unit	Scanning method	Galvanometer mirror scanners (both X and Y)
	Field number	20 (10 with use of LD405 laser)
	Pinhole	5-position pinhole turret
	Image memory and	Standard scanning mode: 256 x 256 (0.45s) - 2048 x 2048 (10.835s)
	scanning speed	Bi-directional high-speed scanning mode: 512 x 512 (0.25s) (Simultaneous scanning of up to 2 channels)
	Image channel	Selectable from 2-channel (fluorescence) or 2-channel (fluorescence) + 1-channel (transmitted light)
		3-channel (fluorescence) using virtual channel
	Selection of filters according to staining	Manual selection
	Scanning modes	XY, XYZ, XYT, XYZT, XZ, XT, XZT, point, Line-t, free line-t, line-z, free line-z, Clip, ZoomIn
	Image depth resolution	12-bit (=4096 grey levels)
	Zoom	1X-10X
	Z-drive	Step motor/Minimum step 10nm (BX61, BX61WI and IX81 combination), 25nm (other microscope combination)
Microscopes	Upright	BX51, BX61, BX51WI, BX61WI
	Inverted (special laser safe frame)	IX81FVSF, IX71FVSF (side port)
External trans-	Transmitted light illumination unit	External halogen light source connected to microscope via fiber cable
mitted light unit	Transmitted light detector	External detector unit with built-in photomultiplier Connected to microscope frame via fiber cable
Fluorescence illumination unit		Connect to external mercury light source and microscope via fiber cable
		Standard equipment of FV300-BX51, FV-300-BX61, FV300-BX51WI, FV300-BX61WI
PC with system control boards		PC-AT compatible machine/OS: Windows XP (English version)/ 1GB memory (can be expanded to a maximum of 4GB)
		CPU: Pentium 4, over 2.8GHz, Special I/F board/image capture: PCI bus
		Graphic board: G450_Dual 32MB
		Hard disk: 80GB 7200rpm_ID (ATA100) with DVD-ROM
		Monitor: Two 19” LCD monitors are recommended, each able to display 1280x1024 images in full color (16.77 million colors)
		LAN: On board
Fluoview	Image acquisition	Scanning condition setting: image size, scanning speed, zoom, panning etc.
application		Real-time image calculation: Kalman filtering, peak integration,
software
	Hardware control	Laser, scanning unit, microscope
		Each image display: Single-channel side-by- side, merge, cropping, tiling, series (Z/T) pass and continuous
	Image display	LUT: Individual color setting, pseudo-color, Overlay: Lines, text, scale bar, etc
	Image processing	Individual filter: Average, Low-pass, High-pass, Sobel, Median, Prewitt, 2D Laplacian, edge enhancement etc.
		Calculations: Inter-image, mathematical and logical,
		DIC back ground leveling
	Image analysis	Overview of fluorescence intensity within an area, histogram, perimeter measurement for user-assigned area,
		time-lapse measurement , etc.
	3D visualization	3D animation, left / right stereo pairs, red / green stereoscopic images and cross section
	Others	Graphic-based help, PAPP (Programmable Acquisition Protocol Processor), time course software (optional),
		trigger IN/OUT function (optional), Multi point software (optional)
Power consumption		Microscope (115V 6A/230V 3A), scanning unit+PSU (115V 3.5A/230V 2A), computer & monitor (115V 4.5A/230V 10A),
		Ar laser (115V 10A/230V 5A), Mult-linei Ar laser (115V 10A/230V 5A), Kr laser (230V 20A),
		HeNe laser each (115V 0.4A/230V 0.2A), LD laser (405nm, 440nm: 100V 0.9A/230V 0.5A)

Objectives for BX and IX

(using U-UCD8, IX-LWUCDA and U-DICTS)

			Cover glass			Condenser for BX	Condenser for IX	U-DICTS
Description	NA	W.D		Immersion	Correction ring	U-UCD8A	IX-LWUCDA
			thickness					position
						optical element	optical element
UPLSAPO 4X	0.16	13	—
UPLSAPO 10X	0.40	3.1	0.17			U-DIC10	IX2-DIC10	normal
UPLAPO 10XO3	0.40	0.24	0.17	Oil		U-DIC10	IX2-DIC10	normal
UPLAPO 10XW3	0.40	0.43	0.17	Water		U-DIC10	IX2-DIC10	normal
UPLSAPO 20X	0.75	0.6	0.17			U-DIC20	IX2-DIC20	normal
UPLAPO 20XO3	0.80	0.19	—	Oil		U-DIC20	IX2-DIC20	normal
UPLSAPO 40X	0.90	0.2	0.11-0.23		_	U-DIC40	IX2-DIC40	normal
UPLFLN 40XO	1.30	0.2	0.17	Oil		U-DIC40	IX2-DIC40	normal
PLAPON 60XO	1.42	0.15	0.17	Oil		U-DIC60	IX2-DIC60	BFP1
UPLSAPO 60XO	1.35	0.15	0.17	Oil		U-DIC60	X2-DIC60	normal
UPLSAPO 60XW	1.20	0.28	0.15-0.2	Water	_	U-DIC60	X2-DIC60	normal
UPLSAPO 100XO	1.40	0.12	0.17	Oil		U-DIC100	X2-DIC100	normal

Objectives for fixed stage upright microscopes (using WI-UCD, WI-DICTHRA)

Objective	N.A.	W.D.	DIC	Revolving
			prism	nosepiece
MPL5X	0.10	19.60	—	WI-SSNP,
				WI-SRE2
UMPLFL10XW	0.30	3.30	U-LDPW10H	WI-SSNP,
				WI-SRE2
UMPLFL20XW	0.50	3.30	U-LDPW20H	WI-SSNP,
				WI-SRE2
LUMPLFL40XW	0.80	3.30	U-LDPW40H	WI-SSNP,
				WI-SRE2
LUMPLFL60XW	0.90	2.00	U-LDPW60H	WI-SSNP,
				WI-SRE2
LUMPLFL40XW/IR	0.80	3.30	U-LDPW40H	WI-SSNP,
				WI-SRE2
LUMPLFL60XW/IR	0.90	2.00	U-LDPW60H	WI-SSNP,
				WI-SRE2
LUMPLFL100XW	1.00	1.50	U-LDPW60H	WI-SSNP,
				WI-SRE2
XLUMPLFL20XW	0.95*	2.00	U-LDPXLU20	WI-SNPXLU
			HR

*Note: These conditions are not met in confocal microscopy

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