UVPS 81-0112-01 Rev C manual Applications

UV CROSSLINKERS UVP

5.APPLICATIONS

The Crosslinker is a multi-purpose ultraviolet exposure instrument for use in the laboratory. A wide variety of applications for ultraviolet radiation exist in the laboratory.

A.UV crosslinking of DNA and RNA by covalently binding nucleic acids to transfer membranes nitrocellulose, nylon or nylon-reinforced nitrocellulose membranes after Northern, Southern, slot or dot blotting.

The PRESET ULTRAVIOLET EXPOSURE SETTING is factory set to a UV dose of 120,000 microjoules per cm² for this laboratory purpose. This setting has been found to be the optimal dose for DNA retention and hybridization-signal sensitivity.

B.Nicking ethidium-bromide stained DNA in Agarose Gels

C.Gene Mapping for creating cleavage-inhibiting thymine dimers

D.Testing RecA function

E.Ultraviolet Sterilization

F.Elimination of PCR contamination

G.UV Curing

H.Fluorescence of materials

Further Applications can be achieved with the Crosslinkers by changing the ultraviolet wavelength (see Section 7 Changing the wavelength).

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