Olympus FV300 manual Applications Gallery, Structure of PtK2 cell

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Applications Gallery

Neuron

Lucifer Yellow: retina ganglion cell

TexasRed: dopamine-operated amacrine cell

Prof. Shigetada Nakanishi

Dept. of Biological Sciences,

Kyoto Univ. Faculty of Medicine

Mouse hippocampal neurons GFP: postsynaptic density protein Rhodamine-phalloidin: actin

Hippocampal neurons expressing a GFP-tagged postsynaptic density protein were fixed and stained with rhodamine-phalloidin to visualize the localization of cytoplasmic actin filaments.

In dendrites, actin filaments are concentrated in the postsynaptic sites.

Shigeo Okabe

Department of Anatomy and Cell Biology

Tokyo Medical and Dental University

Morphology

Purkinje cell in the rat cerebellum

FITC: vesicular GABA transporter VGAT

Cy3: vesicular glutamate transporter VGLUT1

Pr. Masahiko Watanabe

Department of Anatomy,

Hokkaido University School of Medicine

Lucifer yellow injected visual interneurons of swallowtail butterfly

Extended focus is used for every 100µm on 383µm Z-range image and displayed by overlapping pseudo colors Mituyo Kinoshita, Pr. Kentaro Arikawa

Laboratory of Neuroethology,Graduate School of

Integrated Science, Yokohama City University

 

Rat tongue taste bud

 

 

DAPI: Nuclei

 

 

FITC: TrkB, high-affinity receptor for brain-derived

 

 

neurotrophic factor

 

 

Texas Red: Protein Gene Products

 

 

Pr. Shigeru Takami

 

 

Department of Anatomy,

 

 

School of Health Science,

 

 

Kyorin University

 

 

 

 

Structure of PtK2 cell

Human Colon Crypt

Nucleus: DAPI (Blue)

Alexa 488 and To-Pro 3

Actin: FITC (Green)

Christine Anderson,Prof. Ray White's Laboratory,

Mitochondria: Mito Tracker (Red)

Huntsman Cancer Institute, U. Utah

Microtubules: Cy5 (White)

 

 

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Contents FV300 FV300-IX71configuration Page Software Graphical User Interface Easy exchange between display methods Image tool barThumbnail display Scanning unit set-up monitor displayTime Course High-speed 4 frames/sec image acquisitionVersatile line scanning modes have many uses Superior slice patching systemInput/output of external trigger signal 440nm diode laser can be added for CFP/YFP Fret imagingRatio imaging to analyze 2-wavelength images Papp for Frap Application Features Measure up to 254 points Multi-point time lapse systemHigh-precision XY stage scanning Flexible scanning conditionsEasy Z axis operation and setting 3D ImagingThreshold Mode Regions/Min-Max ModeColocalization Link with patch clamping dataApplications Gallery Structure of PtK2 cellFluorescent Proteins 300 400 500 600 Short Pass Band Pass Long PassFluorochrome Specifications ISO9001