Olympus FV300 manual Time Course, High-speed 4 frames/sec image acquisition

Time Course

Using different scanning modes to chart time-lapse changes efficiently.

X -t	Y -t	SlantLine-t	FreeLine-t		X-Y-t
				Calcium wave in Xenopus oocyte, Calcium Green staining, fluorescence
				pseudo-colored fluorescence image after injection of inositol 3-trisphospate
				Japan Science and Technology Corporation, Exploratory Research for Advanced Technology,
X-Y-t	X-Z-t	Y-Z-t	ZoomIn-t
				Mikoshiba cell control project, Prof. Aya Muto

X-Y-Z-t ZoomIn-Z-t

High-speed (4 frames/sec) image acquisition

For the high speed observation of the sample, Fluoview is capable of scanning 4 frames per second in a fast scanning mode at an image size of 512X512. By limiting the image size, the frame rate will be even faster. This scanning mode is suitable for living cell observation.

Versatile line scanning modes have many uses

The wide variety of the line scanning modes (linear/slant/free-line) enables flexible analysis of rapid time-lapse experiments.

X -t X -t

Calcium wave in isolated cardiac myocyte	Calcium sparks in isolated cardiac myocyte
Dr. Sandor Gyorke	Dr. Sandor Gyorke
Texas Technical University	Texas Technical University

Superior slice patching system

In combination with the unique fixed stage & nosepiece focusing BX61WI microscope, the FV300 provides a highly effective system for slice patching. This unique set- up has a small footprint for increased room in a space-limited cage. The remote control microscope options minimize the danger of accidentally touching the delicate experimental settings.Olympus also offers ideal non-cover glass long working distance water immersion objectives and an optional XY translation stage that moves the entire confocal microscope system while the sample and other experimental hardware remains in a fixed position.

Highly precise time-lapse analysis

Fluoview’s wide dynamic range of 12-bit or 4096 grey levels provides enough sensitivity to detect even the slightest changes in intensity. The user can designate multiple regions of interest (ROI) by using drawing tools. The fluorescence intensity or the ratio can be analyzed with the intuitive GUI driven program.

Patch clamp	Image acquisition	ROI designation	Intensity versus time
			measurement

Immersion-type LUMPLFL objectives

The 40X water immersion objective in this series has a 3.3mm working distance and an extremely fine tip which is suitable for micromanipulation using a fixed stage upright microscope. It has a large N.A. (0.8) and is also ideal for confocal observations. When using the BX61WI fixed stage & nosepiece focusing upright microscope with water immersion objectives, confocal imaging can be used to monitor time-lapse fluorescence changes in thick specimens such as brain slices.

	Long working distance, non-cover
BX61WI fixed stage upright microscope+translation stage	glass water immersion objective

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