Olympus FV300 manual 3D Imaging, Easy Z axis operation and setting

Page 10

3D Imaging

Using multiple 3D images to obtain accurate 3D structure analysis.

X-Y-Z ZoomIn-Z

X -Z

Y -Z

SlantLine-Z FreeLine-Z

Easy Z axis operation and setting

The upper and lower limit of Z scanning can be specified interactively by actually scanning the sample or by direct input of the numerical value.

X-Y-Z

Tile display

Acquire X-Y-Z images and display X-Y cross-sectional images quickly and continuously in increments of 0.01*µm

Thanks to the precision driving mechanism that enables 0.01µm step control within the BX61, BX61WI and IX81 motorized microscopes, high-quality continuous cross-sectional images can be acquired. The 3D function also provides extended focus projections, red/green stereo views, topographic projections and 3D animations for exploring the structure of the sample. Multi-plane images can be created from an XYZ image series, enabling easy

measurement and observation of horizontal X-Zand vertical cross sections. Other useful procedures include 3D image cropping,

series animation and simple volume measurement.

*0.025µm is the smallest increment for other microscope combinations.

Y -Z

Y-Z

X -Z

Topographic projection

Height of 3D structure indicated by color.

9

Image 10
Contents FV300 FV300-IX71configuration Page Software Graphical User Interface Thumbnail display Easy exchange between display methodsImage tool bar Scanning unit set-up monitor displayVersatile line scanning modes have many uses Time CourseHigh-speed 4 frames/sec image acquisition Superior slice patching system440nm diode laser can be added for CFP/YFP Fret imaging Input/output of external trigger signalRatio imaging to analyze 2-wavelength images Papp for Frap Application High-precision XY stage scanning Features Measure up to 254 pointsMulti-point time lapse system Flexible scanning conditionsEasy Z axis operation and setting 3D ImagingColocalization Threshold ModeRegions/Min-Max Mode Link with patch clamping dataApplications Gallery Structure of PtK2 cellFluorescent Proteins Short Pass Band Pass Long Pass 300 400 500 600Fluorochrome Specifications ISO9001