Olympus FV300 manual Papp for Frap Application

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PAPP for FRAP Application

PAPP: Programmable Acquisition Protocol Processor

Easy, reliable flow of experiments for fluorescence recovery after photobleaching.

AOTF and PAPP function for effective FRAP (Fluorescence Recovery After Photobleaching)

Fluorescence recovery after photobleaching can be analyzed on any designated area by means of the AOTF-equipped laser combiner. During the processes of photobleaching and recovery, the PAPP function enables time scales to be freely and easily programmed to suit different experiment purposes.

Mouse; hippocampal neurons; fluorescence of GFP

Living neurons expressing GFP were maintained in culture and fluorescent images were obtained. Subsequently, FRAP analysis was performed on the same cell to determine the diffusion rate of GFP proteins into the dendritic spines. Rapid fluorescence recovery ( within seconds ) was observed.

Shigeo Okabe

Department of Anatomy and Cell Biology

Tokyo Medical and Dental University

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q Fluorescence Baseline w - t

Photobleaching at 0.22 sec interval y - o

Fluorescence recovery at 0.32 sec interval

Fluorescence quantitation Line graph depicting average fluorescence intensity versus time.

New PAPP (Programmable Acquisition Protocol Processor) makes it easy to program a wide range of experiments

Using the new PAPP function, which is included in standard software, the experiment protocol is created by describing the individual steps or phases within the experiment. Users can specify detailed conditions and parameters for each step. This function enables users to construct complex experimental protocols with minimal effort. PAPP is suitable, for example, for FRAP experiments that require more flexibility.

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Contents FV300 FV300-IX71configuration Page Software Graphical User Interface Easy exchange between display methods Image tool barThumbnail display Scanning unit set-up monitor displayTime Course High-speed 4 frames/sec image acquisitionVersatile line scanning modes have many uses Superior slice patching systemRatio imaging to analyze 2-wavelength images Input/output of external trigger signal440nm diode laser can be added for CFP/YFP Fret imaging Papp for Frap Application Features Measure up to 254 points Multi-point time lapse systemHigh-precision XY stage scanning Flexible scanning conditionsEasy Z axis operation and setting 3D ImagingThreshold Mode Regions/Min-Max ModeColocalization Link with patch clamping dataApplications Gallery Structure of PtK2 cellFluorescent Proteins Fluorochrome 300 400 500 600Short Pass Band Pass Long Pass Specifications ISO9001