Multi-point time lapse system

High-magnification multi-point time lapse observation of living cells.

Introduction of DsRed2 expression vector into brain of 14th day mouse embryo through womb electroporation. Taking a specimen slice from the 15th day embryo and observing the living nerve stem cell and nerve cells subsequently born from it.

Wide-ranging specimen observations for improved experiment throughput

Use of a motorized XY stage allows the analysis of time lapse changes in many points scattered over a wide area. The system is therefore effective for work with thick specimens, such as observing changes in the states and movements of stem cells using a brain slice, or analyzing expression mechanisms at the individual level in an embryo. In wide-ranging tasks such as analyzing cell functions using GFP, the system provides many different kinds of data at the same time, enabling a higher overall level of experiment efficiency even in long-lasting observations. In addition, using separate chambers at the same time makes it possible to perform different experiments at the same time. These are just some of the ways in which this system dramatically improves the throughput of experiments requiring long-duration observations.

Image courtesy of:

Dr. Kazunori Nakajima

Dr. Hidenori Tabata

School of Medicine, Keio University

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Using multi-point software*, it is possible to acquire an XYt, an XYZ or and XYZt image series at multiple positions through automated software control of the motorized XY stage.

Features

1. Measure up to 254 points

A variety of scan conditions can be set for each point, such as XYZ coordinates, the Z-axis acquisition range and the detector sensitivity.

2.Up to 5 X 5 adjacent fields of view registered automatically

Since adjacent fields of view are registered automatically, it is possible to broaden any given field while maintaining a high magnification level.

3.High-precision XY stage scanning

A wide area can be observed with highly precise position reproduction. Errors from repetition are not accumulated. (High-precision XY stage complies exclusively with the "PROSCAN" model from PRIOR Scientific)

4. Flexible scanning conditions

The system combines scan flexibility with time lapse imaging for monitoring changes in the specimen over time.

* Multi-point software and motorized XY stage are optional