Olympus manual FV1000MPE Multiphoton Excitation System

Technology / Hardware

Exceptional Resolution for Imaging of Cytoplasmic Membrane and Areas Deep Within Living Specimen.

TIRFM (Total Internal Reflection Fluorescence Microscope) System

Switchable between Confocal and TIRFM Imaging

Switchable between confocal and TIRFM imaging for localization of proteins on the cytoplasmic membrane surface and acquisition of sectioning images within cells.

Software Control of TIRF Illumination

Built-in laser provides TIRF illumination. Software can be used to tune the angle of incidence of excitation light and calculates the penetration depth of the evanescent wave based on the TIRF objective used.

High-Numerical Aperture Objectives for TIRF Illumination
A line of high-numerical aperture (NA) objectives is available for
TIRF illumination.					TIRFM	LSM
					GFP—Pak—K298A in HeLa cells.
					Courtesy of Dr.J M Dong of sGSK-NRP laboratory, Singapore
NEW	APON60xOTIRF	NEW	UAPON100xOTIRF	NEW	UAPON150xOTIRF	Apo100xOHR
	NA : 1.49 (oil immersion)		NA : 1.49 (oil immersion)		NA : 1.45 (oil immersion)	NA : 1.65 (oil immersion)
	WD: 0.1 mm		WD: 0.1 mm		WD: 0.08 mm	WD: 0.1 mm
						(Customized cover glass and
						immersion oil)

FV1000MPE Multiphoton Excitation System

Brighter and Deeper Imaging with Finer Resolution

The FV1000 is upgradeable to multiphoton excitation capability by adding a dedicated laser and multiphoton optical system. Optical design is optimized for multiphoton principles for brighter imaging of features deep within living specimens, at higher resolutions than previously possible.

Special Multiphoton Objective with Outstanding

Brightness and Resolution

Olympus offers a high NA water-immersion objective designed for a wide field of view, with improved transmittance at near- infrared wavelengths. A correction collar compensates for spherical aberration caused by differences between the refractive indices of water and specimens, forming the optimal focal spot even in deep areas, without loss of energy density. The objective is designed to collect scattered light over a wide field of view for maximum image brightness.

XLPLN25xWMP
Magnifications	: 25x
NA	: 1.05 (water immersion)
W.D.	: 2.0 mm

Multiphoton Laser Light Stimulation

Adding a multiphoton laser to the SIM scanner enables multiphoton laser light stimulation or uncaging confined to the focal volume.

3-dimensionally constructed images of neurons expressing EYFP in the cerebral neocortex of a mouse under anesthesia.

Courtesy of:

Hiroaki Waki, Tomomi Nemoto, and Junichi Nabekura

National Institute for Physiological Sciences, National Institutes of Natural Sciences, Japan

* The FLUOVIEW FV1000MPE is a class 4 laser product.

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