Olympus FV1000 manual Expandability to Support Diverse Application, Analysis

Expandability to Support Diverse Application.

				Application		Standard Functions	Optional Functions
Molecular interaction and							Intracellular diffusion measurement
molecular concentration							Calculation of diffusion coefficients for intracellular molecules, and analysis of
analysis						—	molecular binding and changes in molecular density.
							Supports a wide range of methods (RICS/ccRICS, point FCS/point FCCS and
1.5							FRAP).
1							Software Required: Diffusion measurement package
0.5				130
					125 Pixels
0
105
125				105
			Pixels	130
Laser Light stimulation						Acquires images while rapidly switching	SIM scanner system
						the built-in laser between imaging and	Performs simultaneous imaging and laser light stimulation. Provides detailed
						laser light stimulation.	settings for laser light stimulation including position and timing.
						Features tornado scanning for high-	Features tornado scanning for high-efficiency bleaching using laser light
						efficiency bleaching using laser light	stimulation.
						stimulation.	Equipment Required: SIM scanner, laser combiner (dual fiber version)
							Multi-point laser light stimulation system
							Register multiple points for laser light stimulation, and program the respective
							stimulation order, stimulation time and type of stimulation (continuous laser light
							or pulse laser light).
							Software Required: Multi-stimulation software
Multi-dimensional							Long time-lapse system
time-lapse imaging							Microscopes equipped with zero drift compensation (ZDC) acquire each image at
							a set focus plane. The microscope CO2 incubator maintains cell activity for a long
			P2
	P1				P3		period for continuous imaging.
		P5		P4		—	Equipment Required: IX81-ZDC microscope, CO2 incubator
							Multi-point scanning system
							Register multiple points for repeated image acquisition. Efficiently observe multiple
							cells in parallel on 35-mm dishes, microplates or chamber slides.
							Software and Equipment Required:
							Multi-area time-lapse software, motorized XY stage**
3D mosaic imaging							3D mosaic imaging system
							Continuous imaging of adjacent fields of view and mosaic imaging to form a
						—	composite image.
							Acquisition of adjacent Z-series images for 3D mosaic imaging.
							Software and Equipment Required:
							Multipoint time-lapse software, motorized XY stage**
TIRFM							TIRFM imaging
							Uses the laser from the laser combiner to provide evanescent illumination, for
						—	imaging the movement of molecules near the glass surface, such as cell
							membranes and adhesion factors.
							Software and Equipment Required: TIRFM unit*, TIRF objective, high-
							sensitivity CCD camera**, CCD camera control software**
FRET						Provides FRET analysis functions.	CFP-YFP FRET
						Diode laser offers exceptional stability	Ratio imaging and sensitized emission.
						and long life.	Available 440 nm diode laser is optimized for CFP-YFP FRET experiments
						Supports FRET efficiency	methods.
						measurements using acceptor	Diode laser offers exceptional stability and long life.
						photobleach method.	Equipment Required: LD 440 nm Laser
Multi-color imaging						Three-channel detector for	Imaging blue dyes
						simultaneous acquisition of	Available 405-nm laser for image acquisition of multi-stained samples labeled with
						fluorescence images from three	V-excitation fluorescent dyes such as DAPI, Hoechst and Alexa 405.
						different dyes.	Equipment Required: LD 405 nm laser
						Sequential mode for acquisition of
						fluorescence images without cross-talk. Simultaneous four-color imaging
						Fluorescence can also be separated	Fourth channel detector can be easily added to simultaneously acquire images of
							four colors.
						using unmixing (only available on
							Equipment Required: 4-channel detector
						spectral scan unit).
Colocalization analysis						Easily determine if labeled substances	High-accuracy colocalization analysis
						are present locally in the same	New 60x oil-immersion objective offers image acquisition with exceptional
						locations.	positional accuracy coefficient.
						Calculate of Pearson coefficients,	Equipment Required: PLAPON 60xOSC
						overlap coefficients and colocalization
						indices.

*SIM scanner and TIRFM scanner cannot be installed on the same system.

**For more information about peripheral equipment, contact your Olympus dealer.

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