Olympus FV1000 manual Excellent Precision, Sensitivity and Stability, Laser combiner/Fiber

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Excellent Precision, Sensitivity and Stability.

FLUOVIEW Enables Precise, Bright Imaging with Minimum Phototox

 

 

Main scanner

 

 

 

 

Barrier filter

 

 

 

 

 

 

 

 

Grating

 

 

 

 

 

 

 

Laser combiner

 

Grating

 

 

 

 

 

 

 

 

 

 

LD635

 

Broadband fiber *

 

 

 

 

Confocal pinhole

 

 

 

 

 

LD559

AOTF

LD473

LD405

AOTF

Broadband fiber

Laser combiner/Fiber

Diode Laser

Greater stability, longer service life and lower operating cost are achieved using diode lasers.

Laser Feedback Control

Scanner unit is equipped with laser power monitor for feedback control enhancing stable laser output.

Laser Compatibility

Diode laser :

405 nm, 440 nm, 473 nm, 559 nm, 635 nm

Gas laser :

Multi-line Ar laser (458 nm, 488 nm, 515 nm) HeNe(G) laser (543 nm)

Broadband Fiber

Broadband fiber connection for 405–635 nm lasers, to achieve an ideal point light source with minimal color shift and position shift between images.

Laser Combiner

Two versions available.

•Single fiber-type combiner is used for main scanner FV1000 with up to six lasers, ranging from 405 to 635 nm.

•Dual fiber-type combiner is used for laser light stimulation with main and SIM scanner FV1000.

Scanners/Detection

High Sensitivity Detection System

High-sensitivity and high S/N ratio optical performance is achieved through the integration of a pupil projection lens, use of a high sensitivity photomultiplier tube and an analog processing circuit with minimal noise. Enables high S/N ratio image acquisition with minimal laser power to reduce phototoxicity.

Up to Four PMT Channels

Three integrated confocal PMT detectors, and optional module with fourth confocal PMT expandable up to four PMT channels.

Spectral Scanning Unit

Filter Scanning Unit

Two Versions of Light Detection System

Spectral detection for high-precision spectroscopy with 2 nm resolution.

Filter detection equipped with high quality filter wheels.

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Contents FV1000 FLUOVIEW--From Olympus is OpenImaging to Analysis Ing up New WorldsAdvanced Fluoview Systems Enhance the Power of Your Research Page Excellent Precision, Sensitivity and Stability Laser combiner/FiberScanners/Detection Icity Optical SystemSamples and Specimens Spectral Based Detection Filter Based DetectionWide Choice of Bleaching Modes SIM Simultaneous Scanner UnitSimultaneous Laser Light Stimulation and Imaging Multi-Purpose Laser CombinerBest Reliability for Colocalization Analysis Improved Flatness and Resolution at 405 nmNEW Low Chromatic Aberration Objective Flatness Comparison Image at 1x ZoomFV1000MPE Multiphoton Excitation System User-Friendly Software to Support Your Research Multi Stimulation Software Re-Use FunctionHelp Guide Multi-Area Time-Lapse SoftwareFret Measurement Multi-Dimensional Time-Lapse3D/4D Volume Rendering Light StimulationPoint FCS-Point scan Fluorescence Correlation Spectroscopy Diffusion Measurement PackageRICS-Raster Imaging Correlation Spectroscopy Frap AnalysisRics Analysis Method Rics Application and PrinciplesRics Principle Spatial Correlation AlgorithmFRAP-Fluorescence Recovery after Photobleaching Laser Light StimulationFLIP-Fluorescence Loss in Photobleaching Photoconversion UncagingMulti-Point Laser Light Stimulation Focal Drift Compensation for Long Time-Lapse Imaging Multi-Dimensional Time-LapseSignificantly Improved Long Time-Lapse Throughput Maintain Cell Activity Over a Long Period3D Mosaic Imaging Mosaic Imaging for 3D XYZ ConstructionAutomated from 3D Image Acquisition to Mosaic Imaging Expandability to Support Diverse Application AnalysisNHeNe Ree MCherry405 Ulti 458 473 488 515Laser Systems Optional Upgrade Equipments for FV1000Scanning Units Illumination UnitsFV1000 System Diagram CO2 IncubatorHigh-Precision Motorized Stage/ Prior H117 Using WI-UCD, WI-DICTHRA2 Main SpecificationsUsing U-UCD8A-2, IX2-LWUCDA2 and U-DICTS Objectives for BX2Dimensions, Weight and Power Consumption Recommended FV1000 system setup IX81, BX61, BX61WIImages are courtesy of the following institutions Fluoview website Olympus Corpoaration is ISO9001/ISO14001 certified