Olympus FV1000 manual Scanning Units, Laser Systems, Illumination Units

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Scanning Units

Two types of scanning units, filter-based and spectral detection, are provided. The design is all-in-one, integrating the scanning unit, tube lens and pupil projection lens. Use of the microscope fluorescence illuminator light path ensures that expandability of the microscope itself is not limited. Visible, UV and IR laser introduction ports are provided, as well as a feedback control system.

Scanning Unit for IX81 Inverted Microscope

Scanning Unit for BX61/BX61WI Upright Microscopes

Dedicated mirror unit cassette is required.

Fluorescence illuminator integrated with scanning unit.

Laser Systems

The multi-combiner enables combinations with all of the following diode lasers: 405 nm, 440 nm, 473 nm, 559 nm and 635 nm. The system can also be equipped with conventional Multi-line Ar laser and HeNe(G) laser.

Dual Type

The multi-combiner outputs laser light with two fibers. Light can be used both for observation and laser light stimulation.

Single Type

Single channel laser output. AOTF is standard equipment.

Illumination Units

Conventional illumination modules are designed for long-duration time-lapse experiments. Since light is introduced through fiber delivery systems, no heat is transferred to the microscope.

Fluorescence Illumination Unit

Stand with Mercury lamp house, motorized shutter, and fiber delivery system for conventional fluorescence observation. Light introduction via fiber optic port.

Transmitted Light Detection Unit

External transmitted light photomultiplier detector and 100 W Halogen conventional illumination, integrated for both laser scanning and conventional transmitted light Nomarski DIC observation. Motorized exchange between transmitted light illumination and laser detection. Simultaneous multi-channel confocal fluorescence image and transmitted DIC acquisition enabled.

Optional Upgrade Equipments for FV1000

4th Channel Detector Unit

Attaches to the optional port of either the filter or spectral type scanning unit and is used as a 4th confocal fluo- rescence detection channel. This is a filter-based fluorescence detection unit.

SIM Scanner

Second scanner dedicated for laser light stimulation, synchronized to the FV1000 main scanner for simultaneous laser light stimulation and confocal image acquisition. Independent fiber optic laser introduction port. Dichromatic mirror within motorized optical port of the scan unit required for introduction of laser into main scanner.

TIRFM Unit

Enables control of the necessary volume of excitation light using FV1000 soft- ware. This unit enables TIRF imaging using the laser light source used with Confocal.

Fiber Port for Fluorescence Output

Confocal fluorescence emission can be introduced via fiber delivery system into external device. Fiber port equipped with FC connector (fiber delivery system not included).

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Contents FV1000 FLUOVIEW--From Olympus is OpenImaging to Analysis Ing up New WorldsAdvanced Fluoview Systems Enhance the Power of Your Research Page Excellent Precision, Sensitivity and Stability Laser combiner/FiberScanners/Detection Icity Optical SystemSamples and Specimens Spectral Based Detection Filter Based DetectionSIM Simultaneous Scanner Unit Simultaneous Laser Light Stimulation and ImagingWide Choice of Bleaching Modes Multi-Purpose Laser CombinerImproved Flatness and Resolution at 405 nm NEW Low Chromatic Aberration ObjectiveBest Reliability for Colocalization Analysis Flatness Comparison Image at 1x ZoomFV1000MPE Multiphoton Excitation System User-Friendly Software to Support Your Research Re-Use Function Help GuideMulti Stimulation Software Multi-Area Time-Lapse SoftwareFret Multi-Dimensional Time-Lapse 3D/4D Volume RenderingMeasurement Light StimulationDiffusion Measurement Package RICS-Raster Imaging Correlation SpectroscopyPoint FCS-Point scan Fluorescence Correlation Spectroscopy Frap AnalysisRics Application and Principles Rics PrincipleRics Analysis Method Spatial Correlation AlgorithmFRAP-Fluorescence Recovery after Photobleaching Laser Light StimulationFLIP-Fluorescence Loss in Photobleaching Photoconversion UncagingMulti-Point Laser Light Stimulation Multi-Dimensional Time-Lapse Significantly Improved Long Time-Lapse ThroughputFocal Drift Compensation for Long Time-Lapse Imaging Maintain Cell Activity Over a Long Period3D Mosaic Imaging Mosaic Imaging for 3D XYZ ConstructionAutomated from 3D Image Acquisition to Mosaic Imaging Expandability to Support Diverse Application AnalysisMCherry 405 UltiNHeNe Ree 458 473 488 515Optional Upgrade Equipments for FV1000 Scanning UnitsLaser Systems Illumination UnitsFV1000 System Diagram CO2 IncubatorHigh-Precision Motorized Stage/ Prior H117 Main Specifications Using U-UCD8A-2, IX2-LWUCDA2 and U-DICTSUsing WI-UCD, WI-DICTHRA2 Objectives for BX2Dimensions, Weight and Power Consumption Recommended FV1000 system setup IX81, BX61, BX61WIImages are courtesy of the following institutions Fluoview website Olympus Corpoaration is ISO9001/ISO14001 certified