Olympus FV1000 manual 3D Mosaic Imaging, Mosaic Imaging for 3D XYZ Construction

Application/ Molecular Interaction Analysis

3D Mosaic Imaging

Mosaic imaging is performed using a high-magnification objective to acquire continuous 3D (XYZ) images of adjacent fields of view using the motorized stage, utilizing proprietary software to assemble the images. The entire process from image acquisition to tiling can be fully automated.

Mosaic Imaging for 3D XYZ Construction

Composite images are quickly and easily prepared using the stitching function, to form an image over a wide area. 3D construction can also be performed by acquiring images in the X, Y and Z directions. Tiled images can be enlarged in sections without losing resolution.

CNS markers in normal mice
Objective	: PLAPON60x
Zoom	: 2x

Image acquisition numbers (XY): 32 x 38, 48 slices for each image

Courtesy of: Dr. Mark Ellisman PhD, Hiroyuki Hakozaki, MS Mark Ellisman

National Center for Microscopy and Imaging Research (NCMIR),

University of California, San Diego

Automated from 3D Image Acquisition to Mosaic Imaging

Multi-area time-lapse software automates the process from 3D image acquisition (using the motorized XY stage) to stitching. The software can be used to easily register wide areas, and the thumbnail display provides a view of the entire image acquired during the mosaic imaging process.

Coordinate Information

Thumbnail

20